Volume Measurement

Thomas Davis1, Andrew Zanella1

1 W.M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges, Claremont, California
Publication Name:  Current Protocols Essential Laboratory Techniques
Unit Number:  Unit 1.1
DOI:  10.1002/9780470089941.et0101s10
Online Posting Date:  May, 2015
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Abstract

This unit describes the common types of volumetric apparatus used in the life science laboratory, their use, and care. When an experimenter needs to prepare solutions at accurate concentrations and quantitatively transfer samples of liquid from one container to another, an array of glassware and plasticware is available for these operations ranging from a microliter to more than a liter in volume. Considerations of temperature, solvent compatibility, and safety also need to be taken into account. © 2015 by John Wiley & Sons, Inc.

     
 
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Table of Contents

  • Overview and Principles
  • Micropipettors
  • Pipets
  • Volumetric Containers
  • Burets and Graduated Cylinders
  • Cleaning Volumetric Apparatus
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
  Burfield, J. and Hefter, G. 1987. Oven drying of volumetric glassware. J. Chem. Educ. 64:1054.
  Bykowski, T. and Stevenson, B. 2008. Aseptic Technique. Curr. Protoc. Essen. Lab. Tech. 00:4.1.1‐4.1.11.
  Guzy, M. 2008. Weight measurement. Curr. Protoc. Essen. Lab. Tech. 00:1.2.1‐1.2.11.
  Hughes, J.C. 1959. Testing of glass volumetric apparatus. National Bureau of Standards Circular 602.
  Kuslich, C.D., Chui, B. and Yamashiro, C.T. 2008. Overview of PCR. Curr. Protoc. Essen. Lab. Tech. 00:10.2.1‐10.2.31.
  Lide, D. (Ed.) 1996‐1997. Variation in the density of water with temperature. CRC Handbook of Chemistry and Physics 77:6‐10, 8‐10.
Internet Resources
  http://www.currentprotocols.com/protocol/eta01a
  APPENDIX 1A of Current Protocols Essential Laboratory Techniques Online.
  http://us.mt.com/us/en/home/supportive_content/specific_overviews.Rainin_Literat.oneColEd.html
  Sites for further information:
  http://www.corning.com/lifesciences/us_canada/en/technical_resources/doc_library/index.aspx
  Rainin Web site.
  http://csmedia2.corning.com/lifesciences/media/pdf/productselectionguide_6_03_liquidhandl_cls_lh_333.pdf
  Corning Web sites.
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