Measurement of pH

Emily A. Wiley1, Deb N. Chakravarti2, Kristin M. Slade1

1 Joint Science Department, Claremont McKenna, Pitzer, and Scripps Colleges, Claremont, California, 2 Proteomics Center, Keck Graduate Institute of Applied Life Sciences, Claremont, California
Publication Name:  Current Protocols Essential Laboratory Techniques
Unit Number:  Unit 3.2
DOI:  10.1002/9780470089941.et0302s03
Online Posting Date:  June, 2010
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Abstract

The measurement of pH is one of the most basic and necessary skills in a life science laboratory. The function and physical characteristics of biological molecules are highly sensitive to the pH of the environment. Common biological buffers must be prepared with the appropriate pH, usually close to neutral for most biological applications. This unit includes a discussion of the different pH instrumentation, notably pH electrodes. It also relates basic pH measurement theory to critical parameters for the technique, and covers the correct use, handling, and storage of pH instruments. Curr. Protoc. Essential Lab. Tech. 3:3.2.1‐3.2.16. © 2010 by John Wiley & Sons, Inc.

Keywords: electrode; meter; calibration; Nernst; electrical potential

     
 
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Table of Contents

  • Overview and Principles
  • Measuring pH
  • pH Electrode Care
  • Measurement of pH
  • Internet Resources
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Internet Resources
   http://www.pH‐measurement.com/pdf.shtml
  This document provides a broad range of comprehensive, in‐depth explanations on instrumentation, equipment choices, and pH measurement principles.
   http://www.pH‐meter.info
  This site provides excellent explanations about concepts related to pH measurement.
   http://www.coleparmer.com/techinfo/techinfo.asp?htmlfile=pHMeasurement.htm
  This site provides comprehensive information related to pH instrumentation and basic measurement theory.
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