Aseptic Technique

Tomasz Bykowski1, Brian Stevenson1

1 University of Kentucky College of Medicine, Lexington, Kentucky
Publication Name:  Current Protocols Essential Laboratory Techniques
Unit Number:  Unit 4.1
DOI:  10.1002/9780470089941.et0401s00
Online Posting Date:  October, 2008
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Abstract

This unit describes common laboratory procedures that can reduce the risk of culture contamination (sepsis), collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on Petri dishes.

Keywords: sterile technique; sterile work; Bunsen burner; laminar flow; laminar hood

     
 
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Table of Contents

  • Overview and Principles
  • Strategic Planning
  • Safety Considerations
  • Techniques for Maintaining Aseptic Conditions
  • Troubleshooting
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

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