Sean R. Gallagher1

1 UVP, LLC, Upland, California
Publication Name:  Current Protocols Essential Laboratory Techniques
Unit Number:  Unit 5.1
DOI:  10.1002/9780470089941.et0501s9
Online Posting Date:  October, 2014
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Centrifugation is a ubiquitous technique in the life science laboratory that uses specialized equipment (centrifuges) to spin, or centrifuge, samples in rotors, generating centrifugal force for a range of applications that include pelleting of precipitates, isolating organelles, and protein and DNA purification. The unit describes basic applications, definitions, and references, and provides up‐to‐date tables for rotor specifications from major suppliers. © 2014 by John Wiley & Sons, Inc.

Keywords: centrifuge; rotor; analytical centrifugation; differential centrifugation; preparative centrifugation; sedimentation coefficient; ultracentrifugation

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Table of Contents

  • Introduction
  • Definitions
  • Rotors
  • Literature Cited
  • Figures
  • Tables
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PDF or HTML at Wiley Online Library



Literature Cited

Literature Cited
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  Beaumelle, B.D., Gibson, A., and Hopkins, C.R. 1990. Isolation and preliminary characterization of the major membrane boundaries of the endocytic pathway in lymphocytes. J. Cell Biol. 111:1811‐1823.
  Bergeron, J.J.M., Rachubinski, R.A., Sikstrom, R.A., Posner, B.I., and Paiement, J. 1982. Galactose transfer to endogenous acceptors within Golgi fractions of rat liver. J. Cell Biol. 92:139‐146.
  Blobel, G. and Potter, V.R. 1966. Nuclei from rat liver: Isolation method that combines purity with high yield. Science 154:1662‐1665.
  Cameron, R.S. and Castle, J.D. 1984. Isolation and compositional analysis of secretion granules and their membrane subfraction from the rat parotid gland. J. Memb. Biol. 79:127‐144.
  Carlson, S.S., Wagner, J.A., and Kelly, R.B. 1978. Purification of synaptic vesicles from elasmobranch electric organ and the use of biophysical criteria to demonstrate their purity. Biochemistry 17:1188‐1199.
  Castle, J.D. 1998. Overview of cell fractionation. Curr. Protoc. Cell Biol. 0:3.1.1‐3.1.9.
  Griffith, O.M. 1986. Techniques of Preparative Zonal and Continuous Flow Ultracentrifugation. Beckman Instruments, Palo Alto, Calif.
  Haines, J.L., Korf, B.R., Morton, C.C., Seidman, C.E., Seidman, J.G., and Smith, D.R. (eds.) 2014. Current Protocols in Human Genetics. John Wiley & Sons, New York.
  Hubbard, A.L., Wall, D.A., and Ma, A.K. 1983. Isolation of rat hepatocyte plasma membranes: I. Presence of the three major domains. J. Cell Biol. 96:217‐229.
  Huttner, W.B., Schiebler, W., Greengard, P., and DeCamilli, P. 1983. Synapsin I (Protein I), a nerve terminal–specific phosphoprotein: III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation. J. Cell Biol. 96:1374‐1388.
  Leighton, F., Poole, B., Beaufay, H., Baudhuin, P., Coffey, J.W., Fowler, S., and DeDuve, C. 1968. The large‐scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with Triton WR‐1339. J. Cell Biol. 37:482‐513.
  Loh, Y.P., Tam, W.W.H., and Russell, J.T. 1984. Measurement of pH and membrane potential in secretory vesicles isolated from bovine pituitary intermediate lobe. J. Biol. Chem. 259:8238‐8245.
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  Schnaitman, C. and Greenawalt, J.W. 1968. Enzymatic properties of the inner and outer membranes of rat liver mitochondria. J. Cell Biol. 38:158‐175.
  Scott, D.J., Harding, S.E., and Rowe, A.J. (eds.) 2006. Analytical Ultracentrifugation: Techniques and Methods. RSC Publishing, Cambridge, U.K.
  Schuck, P. and Braswell, E.H. 2000. Measuring protein‐protein interactions by equilibrium sedimentation. Curr. Protoc. Immunol. 40:18.8.1‐18.8.22.
  Tuma, P.L. and Hubbard, A.L. 1999. Isolation of rat hepatocyte plasma membrane sheets and plasma membrane domains. Curr. Protoc. Cell Biol. 2:3.2.1‐3.2.16.
  Wattiaux, R., Wattiaux‐DeConnick, S., Ronveaux‐Dupal, M.‐F., and Dubois, F. 1978. Isolation of rat liver lysosomes by isopycnic centrifugation in a metrizamide gradient. J. Cell Biol. 78:349‐368.
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