Overview of Electrophoresis

Sean R. Gallagher1

1 UVP, LLC, Upland, California
Publication Name:  Current Protocols Essential Laboratory Techniques
Unit Number:  Unit 7.1
DOI:  10.1002/9780470089941.et0701s08
Online Posting Date:  February, 2014
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Abstract

This unit is an overview of the subsequent units on protein and nucleic acid electrophoresis separation techniques. Curr. Protoc. Essential Lab. Tech. 8:7.1.1‐7.1.7. © 2014 by John Wiley & Sons, Inc.

Keywords: electrophoresis; SDS‐PAGE; two‐dimensional; agarose; isoelectric focusing; protein; nucleic acid; separation

     
 
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Table of Contents

  • Overview and Principles
  • Strategic Questions
  • General Concepts
  • Visualization of Resolved Molecules
  • Imaging Resolved Molecules
  • LITERATURE CITED
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

LITERATURE CITED
   Adams, L.D. 1996. Two‐dimensional gel electrophoresis using the ISO‐DALT system. Curr. Protoc. Mol. Biol. 20:10.3.1‐10.3.12.
   Adams, L.D. and Gallagher, S. 2004. Two‐dimensional gel electrophoresis. Curr. Protoc. Mol. Biol. 67:10.4.1‐10.4.23.
   Ausubel, F.M. , Brent, R. , Kingston, R.E. , Moore, D.D. , Seidman, J.G. , Smith, J.A. , and Struhl, K. (eds.) 2012. Current Protocols in Molecular Biology. Chapter 7. John Wiley & Sons, Hoboken, N.J.
   Chory, J. and Pollard, J.D. Jr . 1999. Separation of small DNA fragments by conventional gel electrophoresis. Curr. Protoc. Mol. Biol. 47:2.7.1‐2.7.8.
   Finney, M. 2000. Pulsed‐field gel electrophoresis. Curr. Protoc. Mol. Biol. 51:2.5B.1‐2.5B.9.
   Gallagher, S.R. 1999a. Gel electrophoresis of proteins and nucleic acids. In The Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation ( M.C. Flickinger and S.W. Drew , eds.) pp. 900‐909. John Wiley & Sons, New York.
   Gallagher, S.R. 1999b. One‐dimensional electrophoresis using nondenaturing conditions. Curr Protoc. Mol. Biol. 47:10.2B.1‐10.2B.11.
   Gallagher, S.R. 2006. One‐dimensional SDS gel electrophoresis of proteins. Curr. Protoc. Mol. Biol. 75:10.2A.1‐10.2A.37.
   Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680‐685.
   Reynolds, J.A. and Tanford, C. 1970. The gross conformation of protein‐sodium dodecyl sulfate complexes. J. Biol. Chem. 245:5161‐5165.
   Smith, A. and Nelson, R.J. 2004. Capillary electrophoresis of DNA. Curr. Protoc. Mol. Biol. 68:2.8.1‐2.8.17.
   Voytas, D. 2000. Agarose gel electrophoresis. Curr. Protoc. Mol. Biol. 51:2.5A.1‐2.5A.9.
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