Overview of Blotting

Maria Cristina Negritto1, Glenn M. Manthey2

1 Pomona College, Claremont, California, 2 City of Hope, Beckman Research Institute, Duarte, California
Publication Name:  Current Protocols Essential Laboratory Techniques
Unit Number:  Unit 8.1
DOI:  10.1002/cpet.5
Online Posting Date:  November, 2016
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Blotting techniques are among the most common approaches used in a molecular biology laboratory. These techniques, Southern, northern, and immunoblotting, are applicable to a variety of macromolecules including DNA, RNA, and protein, respectively. Each of the techniques are dependent on the ability to resolve the individual macromolecules in a size‚Äźdependant manner, transfer the molecules to a solid support, and finally use a defined probe to detect the specific molecule of interest. The utilization of the blotting technology over the last 30 years has been instrumental to the elucidation of many fundamental biological processes. The continued use of blotting technology holds promise for even greater discovery over the next 30 years. ¬© 2016 by John Wiley & Sons, Inc.

Keywords: Southern; Northern; Western; probe; hybridization; antibody; membrane; blotting; protein blot; DNA blot

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Table of Contents

  • General Overview
  • General Considerations
  • Southern and Northern Blotting
  • Immunoblotting
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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