Dictyostelium discoideum: A Model System for Cell and Developmental Biology

Sabateeshan Mathavarajah1, Ana Flores1, Robert J. Huber1

1 Department of Biology, Trent University, Peterborough, Ontario
Publication Name:  Current Protocols Essential Laboratory Techniques
Unit Number:  Unit 14.1
DOI:  10.1002/cpet.15
Online Posting Date:  November, 2017
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Abstract

The social amoeba Dictyostelium discoideum has long served as a model system for studying fundamental processes in cell and developmental biology. This eukaryotic microbe is also recognized as a model organism for biomedical and human disease research since the genome encodes homologs of genes linked to human disease, such as those linked to cancer and neurodegeneration. Dictyostelium has a unique life cycle composed of a unicellular growth phase and a multicellular developmental phase that is induced by starvation. During its life cycle, Dictyostelium undergoes conserved cellular processes including, but not limited to, cell proliferation, phagocytosis, intercellular signaling, cell adhesion and motility, chemotaxis, and cell differentiation. The history of the organism, the resources available to researchers in the community, and the diverse ways that Dictyostelium is used in the contemporary research lab are discussed. © 2017 by John Wiley & Sons, Inc.

Keywords: Dictyostelium discoideum; growth; development; model system; human disease; host‐pathogen interactions; social evolution

     
 
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Table of Contents

  • Overview and Principles
  • Notable Discoveries Associated With Dictyostelium
  • New Ways Dictyostelium is used Today
  • Resources Available to Dictyostelium Researchers
  • Summary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Literature Cited

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Key References
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  This article describes the features of dictyExpress, a gene expression database for Dictyostelium growth and development.
  This article reports the sequencing of the Dictyostelium genome and includes a discussion of the number of predicted proteins encoded by the genome and enriched gene families.
  Eichinger, L., & Rivero, F. (Eds.) (2013). Dictyostelium discoideum Protocols: Methods in molecular biology. Totowa, NJ: Humana Press.
  This methods book is the go‐to resource for commonly used techniques and approaches in Dictyostelium research.
  Fey, P., Dodson, R. J., Basu, S., & Chisholm, R. L. (2013). One stop shop for everything Dictyostelium: DictyBase and the Dicty Stock Center in 2012. Methods in Molecular Biology, 983, 59–92. doi: 10.1007/978‐1‐62703‐302‐2_4.
  This article describes the features of dictyBase, the online bioinformatics resource for Dictyostelium research, and the Dicty Stock Center, the central repository for Dictyostelium cell lines and expression constructs.
  Raper, K. B. (1935). Dictyostelium discoideum, a new species of slime mold from decaying forest leaves. Journal of Agricultural Research, 50, 135–147.
  This article was the first report in the scientific literature of the isolation and phenotypic characterization of Dictyostelium discoideum.
  Rot, G., Parikh, A., Curk, T., Kuspa, A., Shaulsky, G., & Zupan, B. (2009). dictyExpress: A Dictyostelium discoideum gene expression database with an explorative data analysis Web‐based interface. BMC Bioinformatics, 10, 256. doi: 10.1186/1471‐2105‐10‐265.
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