General Laboratory Safety and Working with Hazardous Chemicals

George Lunn1, Warren Strober2

1 Baltimore, Maryland, 2 National Institute of Allergy and Infectious Diseases, Bethesda, Maryland
Publication Name:  Current Protocols Essential Laboratory Techniques
Unit Number:  Appendix 1A
DOI:  10.1002/9780470089941.eta01as00
Online Posting Date:  October, 2008
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Abstract

Persons carrying out the protocols in this manual may encounter the following hazardous or potentially hazardous materials: (1) radioactive substances; (2) toxic chemicals and carcinogenic, mutagenic, or teratogenic reagents; and (3) pathogenic and infectious biological agents. Most governments regulate the use of these materials; it is essential that they be used in strict accordance with local and national regulations. Cautionary notes are included in many instances throughout the manual, and some specific guidelines are also provided in this appendix. However, users must proceed with the prudence and precautions associated with good laboratory practice, under the supervision of personnel responsible for implementing laboratory safety programs at their institutions and in compliance with designated guidelines of federal, state, and local officials.

Keywords: safety; hazardous chemicals; radioisotopes; biologic agents

     
 
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Table of Contents

  • Hazardous Chemicals
  • Biohazards and Infectious Biological Agents
  • Acknowledgements
  • Literature Cited
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

   Bretherick, L. 1990. Hazards in the Chemical Laboratory, 4th ed. Butterworth‐Heinemann, London.
   Bretherick, L. and Urben, P.G. 1999. Bretherick's Handbook of Reactive Chemical Hazards, 6th ed. Butterworth‐Heinemann, London. [also available on CD‐ROM]
   Forsberg, K. and Keith, L.H. 1999. Chemical Protective Clothing Performance Index, 2nd ed. John Wiley & Sons, New York.
   Forsberg, K. and Mansdorf, S.Z. 2003. Quick selection Guide to Chemical Protective Clothing, 4th ed. John Wiley & Sons, Hoboken, N.J.
   Furr, A.K. 2000. CRC Handbook of Laboratory Safety, 5th ed. CRC Press, Boca Raton, Fla.
   Lewis, R.J., Sr. 2004. Sax's Dangerous Properties of Industrial Materials, 11th ed. John Wiley & Sons, Hoboken, N.J.
   Lunn, G. and Sansone, E.B. 1994. Destruction of Hazardous Chemicals in the Laboratory, 2nd ed. John Wiley & Sons, New York.
   O'Neil, M.J. 2006. The Merck Index, 14th ed. Merck & Co., Whitehouse Station, N.J.
   U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention (CDC) and National Institutes of Health (NIH). 1999. Biosafety in Microbiological and Biomedical Laboratories (BMBL), 4th ed. (J.Y. Richmond and R.W. McKenney, eds.) U.S. Government Printing Office, Washington, D.C. (GPO S/N 017‐040‐00547‐4).
Key References
Internet Resources
  http://www.ilpi.com/msds/index.html
  Where to find MSDSs on the internet. Contains links to general sites, government and nonprofit sites, chemical manufacturers and suppliers, pesticides, and miscellaneous sites.
  http://www.OSHA.gov
  The OSHA Web site. Standards can be accessed by clicking on the link in the right sidebar labeled Standards.
  http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=STANDARDS&p_id=10106
  Text of OSHA Standard 29 CFR 1910.1450: Occupational Exposure to Hazardous Chemicals in Laboratories.
  http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=STANDARDS&p_id=9992
  Table Z‐1 of OSHA Standard 29 CFR 1910.10000, which provides a list of permissible exposure limits (PELs) for air contaminants.
  http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=STANDARDS&p_id=9993
  Table Z‐2 of OSHA Standard 29 CFR 1910.1000, which provides a list of PELs for toxic and hazardous substances.
  http://hazard.com/msds/index.php
  Main site for Vermont SIRI. One of the best general sites to start a search. Browse manufacturers alphabetically (for sheets not in the SIRI collection) or do a keyword search in the SIRI MSDS database. Lots of additional safety links and information.
  http://siri.uvm.edu/msds
  Alternate site for Vermont SIRI.
  http://www.eh.doe.gov/docs/osh_tr/ch5c.html
  DOE OSH technical reference chapter on personal protective equipment.
  http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm
  Online version of the Biosafety in Microbiological and Biomedical Laboratories (BMBL), 4th Ed.
  http://www4.od.nih.gov/oba/
  Homepage for the Office of Biotechnologies, NIH.
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