Overview of Somatic Cell Hybrid Mapping

John J. Wasmuth1

1 University of California, Irvine, California
Publication Name:  Current Protocols in Human Genetics
Unit Number:  Unit 3.1
DOI:  10.1002/0471142905.hg0301s00
Online Posting Date:  May, 2001
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This unit provides an introduction to the various types of hybrid panels in use and reviews the status of commercially available panels.

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Table of Contents

  • Description of Mapping Methods
  • Characterization and Stability
  • Mapping Methods
  • Commercial Mapping Panels
  • Literature Cited
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Literature Cited

Literature Cited
   Carlock, L.R., Smith, D., and Wasmuth, J.J. 1986. Genetic counterselection procedure to isolate interspecific cell hybrids containing single human chromosomes: Construction of cell hybrids and recombinant DNA libraries specific for human chromosomes 3 and 4. Somatic Cell Mol. Genet. 12:163‐174.
   Cirullo, R.E., Arrendondo‐Vega, F.X., Smith, M., and Wasmuth, J.J. 1983. Isolation and characterization of interspecific heat‐resistant hybrids between a temperature sensitive Chinese hamster cell asparaginyl‐tRNA synthetase mutant and normal human leukocytes: Assignment of the human asnS gene to chromosome 18. Somatic Cell Genet. 9:215‐233.
   Cirullo, R.E. and Wasmuth, J.J. 1984. Assignment of the human mars gene, encoding methionyl‐tRNA synthetase, to chromosome 12 using human x Chinese hamster cell hybrids. Somatic Cell Mol. Genet. 10:225‐234.
   Cox, D.R., Burmeister, M., Price, E.R., Kim, S., and Myers, R.M. 1990. Radiation hybrid mapping: A somatic cell genetic method for constructing high resolution maps of mammalian chromosomes. Science 250:245‐250.
   Dana, S. and Wasmuth, J.J. 1982. Linkage of the leuS, emtB, and chr genes on chromosome 5 in humans and expression of human genes encoding protein synthesis components in human‐Chinese hamster hybrids. Somatic Cell Genet. 8:245‐264.
   Drwinga, H.L., Toji, L.H., Kim, C.H., Greene, A.H., and Mulivor, R.A. 1993. NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics 16:311‐314.
   Dubois, B.L. and Naylor, S.L. 1993. Characterization of NIGMS human/rodent somatic cell hybrid mapping panel 2 by PCR. Genomics 16:315‐319.
   Overhauser, J., McMahon, J., and Wasmuth, J.J. 1987. Identification of 28 DNA fragments that detect RFLPs in 13 distinct physical regions of the short arm of chromosome 5. Nucl. Acids Res. 15:4617‐4627.
   Warrington, J., Hall, L.V., Hinton, L.M., Miller, J.N., Wasmuth, J.J., and Lovett, M. 1991. A radiation hybrid map of 13 loci on the long arm of chromosome 5. Genomics 11:701‐708.
   Wasmuth, J.J. and Carlock, L.R. 1986. Chromosomal localization of the human gene encoding histidyl‐tRNA synthetase: Clustering of genes encoding aminoacyl‐tRNA synthetases on human chromosome 5. Somatic Cell Mol. Genet. 12:513‐517.
   Weber, J.L., Polymeropoulos, M.H., May, P.E., Kwitek, A.E., Xiao, H., McPherson, J.D., and Wasmuth, J.J. 1991. Mapping of human chromosome 5 microsatellite polymorphisms. Genomics 11:695‐700.
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