Statistical Analysis of Radiation Hybrid Data

Aravinda Chakravarti1

1 University of Pittsburgh, Pittsburgh, Pennsylvania
Publication Name:  Current Protocols in Human Genetics
Unit Number:  Unit 3.4
DOI:  10.1002/0471142905.hg0304s00
Online Posting Date:  May, 2001
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This unit opens with a comparison of radiation hybrid and somatic cell hybrid analyses. Definitions, assumptions, and a mathematical model for radiation hybrid analysis are presented. In addition, parametric and nonparametric methods of data analysis are presented.

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Table of Contents

  • Comparison of Radiation Hybrid and Somatic Cell Hybrid Analyses
  • Definitions and Assumptions in Radiation Hybrid Analysis
  • Mathematical Model for Radiation Hybrids
  • Methods for Radiation Hybrid Data Analysis
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
   Altherr, M.R., Plummer, S., Bates, G., MacDonald, M., Taylor, S., Lehrach, H., Frischauf, A.M., Gusella, J.F., Boehnke, M., and Wasmuth, J.J. 1992. Radiation hybrid map spanning the Huntington disease gene region of chromosome 4. Genomics 13:1040‐1046.
   Aston, C.E. and Chakravarti, A. 1992. The gene order problem when using somatic cell hybrids. Cytogenet. Cell Genet. 59:90‐92.
   Boehnke, M. 1992. Radiation hybrid mapping by minimization of the number of obligate chromosome breaks. Cytogenet. Cell Genet. 59:96‐98.
   Boehnke, M., Lange, K., and Cox, D.R. 1991. Statistical methods for multipoint radiation hybrid mapping. Am. J. Hum. Genet. 49:1174‐1188.
   Burmeister, M., Kim, S., Price, E.R., Delange, T., Tantravahi, U., Myers, R., and Cox, D.R. 1991. A map of the distal region of the long arm of human chromosome 21 constructed by radiation hybrid mapping and pulsed‐field gel electrophoresis. Genomics 9:19‐30.
   Ceccherini, I., Matera, I., Sbrana, M., DiDonato, A., Yin, L., and Romeo, G. 1992. Radiation hybrids for mapping and cloning DNA sequences of distal 16p. Somat. Cell Mol. Genet. 18:319‐324.
   Chakravarti, A. and Reefer, J. 1992. A theory for radiation hybrid (Goss‐Harris) mapping: Application to proximal 21q markers. Cytogenet. Cell Genet. 59:99‐101.
   Cox, D.R., Burmeister, M., Price, E.R., Kim, S., and Myers, R.M. 1990. Radiation hybrid mapping: A somatic cell genetic method for constructing high‐resolution maps of mammalian chromosomes. Science 250:245‐250.
   Cox, D.R., Alonso‐Morales, R., Cordero, E., Kuwana, E., O'Conner, K., Rossi, A., Goold, R.D., Masters, S.B., and Myers, R.M. 1993. A continuous radiation hybrid map of 672 STS markers spanning human chromosome 4. Am. J. Hum. Genet. 53:190.
   Falk, C.T. 1992. Multilocus mapping strategies on chromosome 21 data sets: Comparison of results from family data, radiation hybrids, and somatic cell hybrids. Cytogenet. Cell Genet. 59:119‐121.
   Frazer, K.A., Boehnke, M., Budarf, M.L., Wolff, R.K., Emanuel, B.S., Myers, R.M., and Cox, D.R. 1992. A radiation hybrid map of the region on human chromosome 22 containing the neurofibromatosis type 2 locus. Genomics 14:574‐584.
   Gorski, J.L., Boehnke, M., Reyner, E.L., and Burright, E.N. 1992. A radiation hybrid map of the proximal short arm of the human X chromosome spanning incontinentia pigmenti 1 (IP1) translocation breakpoints. Genomics 14:657‐665.
   Green, P. 1992. Construction and comparison of chromosome 21 radiation hybrid and linkage maps using CRI‐MAP. Cytogenet. Cell Genet. 59:122‐124.
   Lange, K. and Boehnke, M. 1992. Bayesian methods and optimal experimental design for gene mapping by radiation hybrids. Ann. Hum. Genet. 56:119‐144.
   Lawrence, S. and Morton, N.E. 1992. Physical mapping by multiple pairwise analysis. Cytogenet. Cell Genet. 59:107‐109.
   MacCluer, J.W., Chakravarti, A., Cox, D., Bishop, D.T., Bale, S.J., and Skolnick, M.H. (eds.) 1992. Genetic analysis workshop 7: Issues in gene mapping and detection of major genes. Cytogenet. Cell Genet. 59:65‐240.
   Richard, C.W., Withers, D.A., Meeker, T.C., Maurer, S., Evans, G.A., Myers, R.M., and Cox, D.R. 1991. A radiation hybrid map of the proximal long arm of human chromosome 11 containing the multiple endocrine neoplasia type 1 (MEN‐1) and bcl‐1 disease loci. Am. J. Hum. Genet. 49:1189‐1196.
   Ryan, S.G., Dixon, M.J., Nigro, M.A., Kelts, K.A., Markand, O.N., Terry, J.C., Shiang, R., Wasmuth, J.J., and O'Connell, P. 1992. Genetic and radiation hybrid mapping of the hyperekplexia region on chromosome 5q. Am. J. Hum. Genet. 51:1334‐1343.
   Tamari, M., Hamaguchi, M., Shimizu, M., Takayama, H., Hohno, T., Yamaguchi, N., Sugimura, T., Terada, M., and Yokota, J. 1992. Ordering of human chromosome 3p markers by radiation hybrid mapping. Genomics 13:705‐712.
   Warrington, J.A., Hall, L.V., Hinton, L.M., Miller, J.N., Wasmuth, J.J., and Lovett, M. 1991. Radiation hybrid map of 13 loci on the long arm of chromosome 5. Genomics 11:701‐708.
   Warrington, J.A., Bailey, S.K., Armstrong, W., Aprelikova, O., Alitalo, K., Dolganov, G.M., Wilcox, A.S., Sikela, J.M., Wolfe, S.F., Lovett, M., and Wasmuth, J.J. 1992. A radiation hybrid map of 18 growth factor, growth factor receptor, hormone receptor, or neurotransmitter receptor genes on the distal region of the long arm of chromosome 5. Genomics 13:803‐808.
   Washington, S.S., Farr, J.E., Chakravarti, A., and Richard III, C.W. 1993. Radiation hybrid mapping of human chromosome 13. Am. J. Hum. Genet. 53:191.
   Weeks, D.E., Lehner, T., and Ott, J. 1992. Preliminary ranking procedures for multilocus ordering based on radiation hybrid data. Cytogenet. Cell Genet. 59:125‐127.
   Winokur, S. Schutte, B., Weiffenbach, B., Washington, S.S., McElligott, D., Chakravarti, A., Wasmuth, J.J., and Altherr, M.R. 1993. A radiation hybrid map of fifteen loci on the distal long arm of chromosome 4, the region containing the gene responsible for facioscapulohumeral muscular dystrophy. Am. J. Hum. Genet. 53:874‐880.
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