Use of Commercially Available Radiation Hybrid Panels

Bryan M. Dechairo1, Alisoun H. Carey1

1 Oxagen, Ltd., Abingdon, United Kingdom
Publication Name:  Current Protocols in Human Genetics
Unit Number:  Unit 3.5
DOI:  10.1002/0471142905.hg0305s25
Online Posting Date:  May, 2001
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Abstract

Several panels are available for puchase, and this unit provides update information on the use of the three commercially available panels and on the interpretation of mapping results using the Internet.Radiation hybrid panels continue to serve as integral biological reagents in physical mapping projects and positional cloning

     
 
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Table of Contents

  • Commercial Radiation Hybrid Panels
  • Mapping a Single Marker of Unknown Location
  • Building an Ordered Map
  • Submitting Data Via the Internet
  • Interpretation of Results
  • Literature Cited
     
 
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Materials

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Literature Cited

Literature Cited
   Cox, D.R. 1995. Mapping with radiation hybrids. Genome Dig. April:14‐15.
   Cox, D.R., Burmeister, M., Price, E.R., Kim, S., and Myers, R.M. 1990. Radiation hybrid mapping: A somatic cell genetic method for constructing high resolution maps of mammalian chromosomes. Science 250:245‐250.
   Deloukas, P., Schuler, G.D., Gyapay, G., et. al. 1998. A physical map of 30,000 human genes. Science 282:744‐746.
   Hudson, T.J., Stein, L.D., Gerety, S.S., et. al. 1995. An STS‐based map of the human genome. Science. 270:1945‐1954. [With supplementary data from the Whitehead Institute/MIT Center for Genome Research, Human Genetic Mapping Project, Data Release 12 (July 1997)].
   Walter, M.A., Spillet, D.J., Thomas, P., Weissenbach, J., and Goodfellow, P.N. 1994. A method for constructing radiation hybrid maps of whole genomes. Nature Genet. 7:22‐28.
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