Multicolor Fluorescence In Situ Hybridization (FISH) Approaches for Simultaneous Analysis of the Entire Human Genome

Charles Lee1, Willem Rens1, Fengtang Yang2

1 Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, 2 University of Cambridge, Cambridge, United Kingdom
Publication Name:  Current Protocols in Human Genetics
Unit Number:  Unit 4.9
DOI:  10.1002/0471142905.hg0409s24
Online Posting Date:  May, 2001
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The ability to generate chromosome‐specific paints and employ combinatorial labeling strategies has made it possible to differentiate all 24 human chromosomes in a single metaphase spread. Such technology is particularly useful when there is a limited number of metaphase spreads for analyses and when interchromosomal rearrangements are ill‐defined or very complex. There are three systems currently available for simultaneous FISH analysis of all human chromosomes: spectral karyotyping (SKY), Multiplex FISH (M‐FISH), and Rx‐FISH. This overview discusses each of these systems and the recent advances which have made them possible.

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Table of Contents

  • Production of Chromosome‐Specific Paint Probes and Combinatorial Labeling
  • Spectral Karyotyping (SKY) and M‐fish
  • Rx‐FISH
  • Comparative Genomic Hybridization
  • Closing Remarks
  • Figures
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Internet Resources
  Applied Imaging home page.
  Applied Spectral Imaging home page.
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  Metasystems home page.
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