GrailEXP and Genome Analysis Pipeline for Genome Annotation

Edward C. Uberbacher1, Doug Hyatt1, Manesh Shah1

1 Oak Ridge National Laboratory, Oak Ridge, Tennessee
Publication Name:  Current Protocols in Human Genetics
Unit Number:  Unit 6.5
DOI:  10.1002/0471142905.hg0605s39
Online Posting Date:  February, 2004
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Abstract

The Gene Recognition and Analysis Internet Link (GRAIL) is one of the most widely used systems for evaluating the protein‐coding potential of anonymous DNA sequences. This unit describes the use of the XGRAIL and genQuest client‐server applications to locate exons in DNA sequences, to develop gene models, and to search databases for homologs. A support protocol describes how to obtain the GRAIL and genQuest client software by anonymous FTP.

     
 
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Table of Contents

  • Basic Protocol 1: Performing Gene Predictions Using the GrailEXP Web Interface
  • Alternate Protocol 1: Using Genome Analysis Pipeline for Comprehensive Analysis of DNA Sequences
  • Commentary
  • Literature Cited
  • Figures
     
 
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Materials

Basic Protocol 1: Performing Gene Predictions Using the GrailEXP Web Interface

  Materials
  • Any computer workstation (PC, Macintosh, Unix, Linux) with Web access
  • Web browser (e.g., Netscape Navigator, Microsoft Internet Explorer)
  • DNA sequence of interest in Raw or FASTA format
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Figures

Videos

Literature Cited

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