Genetic Analyses on DNA Microarrays

Lincoln D. Stein1

1 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Publication Name:  Current Protocols in Human Genetics
Unit Number:  Unit 11.1
DOI:  10.1002/0471142905.hg1101s24
Online Posting Date:  May, 2001
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Abstract

A great stir has coursed through the genetics community with the advent in 1996 of “chip technologies,” a series of related techniques that allow DNA hybridization‐based studies to be performed with unprecedented speed and parallelism. All chip technologies have in common a microarray, a small glass chip approximately one square centimeter in area, to which nucleotide sequences are bound. Fluorescently labeled nucleic acids are hybridized to the microarray and imaged with a laser scanner or fluorescence microscope. This unit offers an overview of the two dominant technologies, cDNA microarrays and oligonucleotide chips. It concludes with a discussion regarding how well microarrays perform real‐world expression analysis.

     
 
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Table of Contents

  • Chips of a Different Flavor
  • DNA Microassays in Practice
  • Conclusions
  • Acknowledgement
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
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