DNA Vaccination

Jeffrey B. Ulmer1, Rino Rappuoli1

1 Chiron Corporation, Emeryville, California
Publication Name:  Current Protocols in Human Genetics
Unit Number:  Unit 13.2
DOI:  10.1002/0471142905.hg1302s37
Online Posting Date:  August, 2003
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This unit details some of the key methods for setting up and testing DNA vaccines in animal models. The basic procedures are discussed, as well as alternative methods that have been developed over the past several years. The gives step‐by‐step instructions for administering the DNA vaccine via intramuscular injection of the quadriceps muscle, while an alternate procedure details injection of the anterior tibialis.

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Table of Contents

  • Basic Protocol 1: DNAVaccine Administration by Intramuscular Injection of the Quadricpeps Muscles in the Mouse
  • Alternate Protocol 1: DNA Vaccine Administration by Intramuscular Injection of the Anterior Tibialis in the Mouse
  • Reagents and Solutions
  • Commentary
  • Literature Cited
  • Figures
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Basic Protocol 1: DNAVaccine Administration by Intramuscular Injection of the Quadricpeps Muscles in the Mouse

  • Mouse (C57BL/6 and BALB/c strains are commonly used)
  • recipeAnesthetic solution (see recipe)
  • recipeDNA vaccine solution (see recipe; also see )
  • 0.3‐ and 0.1‐ml syringes
  • 26‐G and 28‐G, 0.5‐in. needles
  • Electric clippers
  • Cotton balls or gauze pads
  • Additional reagents and equipment for handling and injection of mice ( appendix 3L)

Alternate Protocol 1: DNA Vaccine Administration by Intramuscular Injection of the Anterior Tibialis in the Mouse

  • Polyethylene tubing, i.d. 0.38‐mm
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Literature Cited

Literature Cited
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