Engineering Large Animal Species to Model Human Diseases

Christopher S. Rogers1

1 Exemplar Genetics, Coralville, Iowa
Publication Name:  Current Protocols in Human Genetics
Unit Number:  Unit 15.9
DOI:  10.1002/cphg.18
Online Posting Date:  July, 2016
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Animal models are an important resource for studying human diseases. Genetically engineered mice are the most commonly used species and have made significant contributions to our understanding of basic biology, disease mechanisms, and drug development. However, they often fail to recreate important aspects of human diseases and thus can have limited utility as translational research tools. Developing disease models in species more similar to humans may provide a better setting in which to study disease pathogenesis and test new treatments. This unit provides an overview of the history of genetically engineered large animals and the techniques that have made their development possible. Factors to consider when planning a large animal model, including choice of species, type of modification and methodology, characterization, production methods, and regulatory compliance, are also covered. © 2016 by John Wiley & Sons, Inc.

Keywords: gene editing; gene targeting; human disease; large animal model; somatic cell nuclear transfer

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Table of Contents

  • Introduction
  • GE Large Animal Models
  • Issues to Consider When Developing a GE Large Animal Model
  • The Potential Impact of a GE Large Animal Model
  • Conclusions
  • Acknowledgements
  • Literature Cited
  • Tables
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Literature Cited

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