Sample Preparation

Kevin J. McKernan1

1 Agencourt Bioscience Corporation, Beverly, Massachusetts
Publication Name:  Current Protocols in Human Genetics
Unit Number:  Unit 16.1
DOI:  10.1002/0471142905.hg1601s40
Online Posting Date:  May, 2004
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Abstract

Today's human genetic studies are requiring an ever‐increasing number of sample manipulations. Although a significant investment of time and money are required, automation can considerably reduce human error and improve sample tracking fidelity when applied to such repetitive projects. This unit presents issues involved in the automation of sample preparation for genomic DNA isolation and PCR.

     
 
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Table of Contents

  • Genomic DNA Isolation
  • PCR Automation
  • Sample Preparation
  • Sample Prep Automation
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Birnboim, H.C. and Doly, J. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513–1523.
   Dean, F.B., Nelson, J.R., Giesler, T.L., and Lasken, R.S. 2001. Rapid amplification of plasmid and phage DNA using Phi 29 DNA polymerase and multiply‐primed rolling circle amplification. Gen. Res. 11:1095–1099.
   Elkin, C., Kapur, H., Smith, T., Humphries, D., Pollard, M., Hammon, N., and Hawkins, T. 2002. Magnetic bead purification of labeled DNA fragments for high‐throughput capillary electrophoresis sequencing. Biotechniques 32:1296–1302.
   Hawkins, T.L., McKernan, K.J., Jacotot, L.B., MacKenzie, J.B., Richardson, P.M., and Lander, E.S. 1997. A magnetic attraction to high‐throughput genomics. Science 276:1887–1889.
   Henco, US patent number 5,057,426.
   Hradecna Z. et al 1998. Microb. Comp. Genomics 3:58.
   Knott, 2002. Suppression of non‐specific nucleic acid amplification. PCT Application WO02/057487.
   Lander et al. 2001. Initial sequencing and analysis of the human genome. Nature 409:860–921.
   Wild, J., Hradecna, Z., and Szybalski, W. 2002. Conditionally amplifiable BACs: Switching from single‐copy to high‐copy vectors and genomic clones. Genome Res. 9:1434–1444.
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