Removal of Lymphoid Organs

J.P. Reeves1, P.A. Reeves1

1 Food and Drug Administration, Bethesda, Maryland
Publication Name:  Current Protocols in Immunology
Unit Number:  Unit 1.9
DOI:  10.1002/0471142735.im0109s01
Online Posting Date:  May, 2001
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Abstract

Although for most experiments lymphoid tissue is removed from the freshly sacrificed animal, there are several circumstances which require the surgical removal, under anesthesia, of either the thymus or spleen, and the recovery of the animal for further investigation. This unit describes survival surgery for removal of the adult spleen as well as the adult and neonatal thymus of the mouse.

     
 
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Table of Contents

  • Basic Protocol 1: Removal of Mouse Lymphoid Organs
  • Key References
  • Figures
     
 
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Materials

Basic Protocol 1: Removal of Mouse Lymphoid Organs

  Materials
  • 70% ethanol
  • Hanks balanced salt solution (HBSS; appendix 2A) or tissue culture medium
  • Iris scissors (straight or angled)
  • Iris forceps (curved)
  • Tissue culture plate
  • Additional reagents and equipment for euthanasia (unit 1.8)
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Figures

Videos

Literature Cited

Key References
   Sjodin, K., Dalmasso, A.P., Smith, J.M., and Martinez, C. 1963. Thymectomy in newborn and adult mice. Transplantation 1:521‐525.
   Van Alten, P.J. 1984. Thymectomy and engraftment of thymic tissue. Methods Enzymol. 108:10‐19.
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