Surgical Removal of Mouse Salivary Glands

Stipan Jonjic1

1 University of Rijeka, Rijeka, Croatia
Publication Name:  Current Protocols in Immunology
Unit Number:  Unit 1.11
DOI:  10.1002/0471142735.im0111s43
Online Posting Date:  August, 2001
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Abstract

Surgical removal of the salivary glands is a simple procedure aimed at providing glandular tissues for studies in histopathology, immunohistology, DNA and RNA analysis, cytokine production, and virus detection and isolation. This unit describes the surgical removal of the salivary glands in mice, but a similar protocol is applicable to rats and other rodents.

     
 
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Table of Contents

  • Commentary
  • Literature Cited
  • Figures
     
 
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Materials

Basic Protocol 1:

  Materials
  • >3‐week‐old mouse
  • 70% ethanol
  • Complete RPMI‐1640, 4°C ( appendix 2A)
  • Operating board
  • Tooth holder: surgical suture or rubber band and dissecting needles
  • Rolled‐up gauze pad
  • Dissection needles
  • Iris forceps with straight or curved ends
  • Iris scissors (straight or curved)
  • Tissue culture plate, on ice
  • Additional reagents and equipment for mouse euthanasia (unit 1.8)
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Figures

Videos

Literature Cited

Literature Cited
  Facini, J.M., Abbot, D.P., and Paulus, G.J.J. (eds.) 1990. Digestive system. In Mouse Histopathology. A Glossary for Use in Toxicity and Carcinogenicity Studies, pp. 72‐74. Elsevier, Amsterdam.
   Jonjic, S., Mutter, W., Weiland, F., Reddehase, M.J., and Koszinowski, U.H. 1989. Site‐directed persistent cytomegalovirus infection after selective long‐term depletion of CD4‐positive T lymphocytes. J. Exp. Med. 169:1199‐1212.
   McQuone, S.J. 1999. Acute viral and bacterial infections of the salivary glands. Otolaryngol. Clin. N. Am. 32:793‐811.
   Mocarski, E.S. 1996. Cytomegaloviruses and their replication. In Fields Virology (B.N. Fields, D.M. Knipe, and P.M. Howley, eds.) pp. 2447‐2492. Lippincott‐Raven, Philadelphia.
   Osborn, J.E. and Walker, D.L. 1971. Virulence and attenuation of murine cytomegalovirus. Infect. Immun. 3:228‐236.
   Polic, B., Hengel, H., Krmpotic, A., Trgovcich, J., Pavic, I., Lucin, P., Jonjic, S., and Koszinowski, U.H. 1998. Hierarchical and redundant lymphocyte subset control precludes cytomegalovirus replication during latent infection. J. Exp. Med. 188:1047‐1054.
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