Techniques in Aseptic Rodent Surgery

Shelley L. Hoogstraten‐Miller1, Patricia A. Brown2

1 National Human Genome Research Institute/National Institutes of Health, Bethesda, Maryland, 2 National Institutes of Health, Bethesda, Maryland
Publication Name:  Current Protocols in Immunology
Unit Number:  Unit 1.12
DOI:  10.1002/0471142735.im0112s82
Online Posting Date:  August, 2008
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Abstract

Performing aseptic survival surgery in rodents can be challenging. This unit describes some basic principles to assist clinicians, researchers, and technicians in becoming proficient in performing aseptic rodent surgery. Curr. Protoc. Immunol. 82:1.12.1‐1.12.14. © 2008 by John Wiley & Sons, Inc.

Keywords: aseptic surgery; technique; rodent; surgical preparation; instrument preparation; suture material; anesthesia; analgesia; surgical gloves

     
 
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Table of Contents

  • Introduction
  • Strategic Planning
  • Basic Protocol 1: Surgical Preparation of the Animal
  • Performing Aseptic Surgery
  • Basic Protocol 2: Performing Aseptic Surgery with Sterile Surgical Gloves
  • Alternate Protocol 1: Performing Aseptic Surgery with Clean Exam Gloves
  • Commentary
  • Literature Cited
  • Figures
     
 
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Materials

Basic Protocol 1: Surgical Preparation of the Animal

  Materials
  • Animal
  • Iodophor or chlorhexidine scrub
  • 70% alcohol
  • Mini‐clipper with no. 0000 blade
  • Adhesive tape (1‐ to 2‐in. wide)
  • 2 × 2–in. gauze pads
  • Cotton‐tipped applicators

Basic Protocol 2: Performing Aseptic Surgery with Sterile Surgical Gloves

  Materials
  • Anesthetized and surgically prepped animal
  • 70% ethanol
  • Surgical attire including:
    • Cap
    • Mask
    • Laboratory coat
  • Sterile surgical instruments and equipment
  • Warming device (see Strategic Planning)
  • Sterile surgical gloves
  • Surgical drape
  • Animal cage
  • Instrument sterilizer

Alternate Protocol 1: Performing Aseptic Surgery with Clean Exam Gloves

  • Clean exam gloves (not sterile surgical gloves)
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Figures

Literature Cited

Literature Cited
   Flecknell, P.A. 1996. Laboratory Animal Anaesthesia, 2nd ed. Academic Press, London.
   Knecht, C.D., Algernon, R.A., Williams, D.J., and Johnson, J.H. 1987. Fundamental Techniques in Veterinary Surgery. WB Saunders Company, Philadelphia, Pennsylvania.
   Kohn, D.H., Wixson, S.K., White, W.J., and Benson G.J. 1997. Anesthesia and Analgesia in Laboratory Animals. Academic Press, San Diego, California.
   National Institutes of Health Animal Research Advisory Committee (NIH ARAC). 2005. Guidelines for Survival Rodent Surgery. (http://oacu.od.nih.gov/ARAC/surguide.pdf)
   National Research Council (NRC). 1996. Veterinary Care. In Guide for the Care and Use of Laboratory Animals (Guide), pp. 556‐570. National Academy Press, Washington, DC. (http://books.nap.edu/readingroom/books/labrats/)
   Swindle, M.M., Vogler, G.A., Fulton, L.K., Marini, R.P., and Popilskis, S. 2002. Preanesthesia, anesthesia, analgesia, and euthanasia. In Laboratory Animal Medicine (J.G. Fox, L.C. Anderson, F.M. Loew, and F.W. Quimby, eds.) pp. 955‐966. Academic Press, San Diego, California.
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