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Techniques in Aseptic Rodent Surgery
Publication Name:
Current Protocols in Immunology
Unit Number:
Unit 1.12
DOI:
10.1002/0471142735.im0112s82
Online Posting Date:
August, 2008
Abstract
Performing aseptic survival surgery in rodents can be challenging. This unit describes some basic principles to assist clinicians, researchers, and technicians in becoming proficient in performing aseptic rodent surgery. Curr. Protoc. Immunol. 82:1.12.1-1.12.14. © 2008 by John Wiley & Sons, Inc.
Keywords: aseptic surgery; technique; rodent; surgical preparation; instrument preparation; suture material; anesthesia; analgesia; surgical gloves
Table of Contents
- Introduction
- Strategic Planning
- Basic Protocol 1: Surgical Preparation of the Animal
- Performing Aseptic Surgery
- Basic Protocol 2: Performing Aseptic Surgery with Sterile Surgical Gloves
- Alternate Protocol: Performing Aseptic Surgery with Clean Exam Gloves
- Commentary
- Literature Cited
- Figures
Materials
Basic Protocol 1: Surgical Preparation of the Animal Materials
Basic Protocol 2: Performing Aseptic Surgery with Sterile Surgical Gloves Materials
Alternate Protocol: Performing Aseptic Surgery with Clean Exam Gloves Additional Materials (also see
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Figures
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Figure 1.12.1 (A) Good surgical site and (B) poor surgical site. -
Figure 1.12.2 Anesthesia machine with precision calibrated vaporizer. -
Figure 1.12.3 Downdraft table. -
Figure 1.12.4 Chemical fume hood. -
Figure 1.12.5 Type IIB biosafety cabinet. -
Figure 1.12.6 Charcoal canister. -
Figure 1.12.7 Simple peel pack. -
Figure 1.12.8 Complex surgical pack. -
Figure 1.12.9 Hot-bead sterilizer. -
Figure 1.12.10 Flash dry-heat sterilizer. -
Figure 1.12.11 Shows the organized surgical instruments. -
Figure 1.12.12 Between surgeries, the tips of the instruments should be covered. -
Figure 1.12.13 Paper surgical drape. -
Figure 1.12.14 Plastic adhesive surgical drape. -
Figure 1.12.15 Sterile gauze pads used for surgical drapes. -
Figure 1.12.16 Sterile tips of the instruments are placed on a sterile field. -
Figure 1.12.17 Arrows indicate space between drape and instruments that is not sterile. -
Figure 1.12.18 A wet area on a paper or cloth drape acts as a wick to pull bacteria through from the nonsterile surface below.
Videos
Literature Cited
| Literature Cited | |
| Flecknell, P.A. 1996. Laboratory Animal Anaesthesia, 2nd ed. Academic Press, London. | |
| Knecht, C.D., Algernon, R.A., Williams, D.J., and Johnson, J.H. 1987. Fundamental Techniques in Veterinary Surgery. WB Saunders Company, Philadelphia, Pennsylvania. | |
| Kohn, D.H., Wixson, S.K., White, W.J., and Benson G.J. 1997. Anesthesia and Analgesia in Laboratory Animals. Academic Press, San Diego, California. | |
| National Institutes of Health Animal Research Advisory Committee (NIH ARAC). 2005. Guidelines for Survival Rodent Surgery. (http://oacu.od.nih.gov/ARAC/surguide.pdf) | |
| National Research Council (NRC). 1996. "Veterinary Care". In Guide for the Care and Use of Laboratory Animals (Guide), pp. 556-570. National Academy Press, Washington, DC. (http://books.nap.edu/readingroom/books/labrats/) | |
| Swindle, M.M., Vogler, G.A., Fulton, L.K., Marini, R.P., and Popilskis, S. 2002. "Preanesthesia, anesthesia, analgesia, and euthanasia". In Laboratory Animal Medicine (J.G. Fox, L.C. Anderson, F.M. Loew, and F.W. Quimby, eds.) pp. 955-966. Academic Press, San Diego, California. | |
