Use of Proteasome Inhibitors

Sondra L. Downey1, Bogdan I. Florea2, Herman S. Overkleeft2, Alexei F. Kisselev1

1 Norris Cotton Cancer Center and Department of Pharmacology and Toxicology, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, 2 Gorlaeus Laboratories, Leiden Institute of Chemistry and Netherlands Proteomics Centre, Leiden
Publication Name:  Current Protocols in Immunology
Unit Number:  Unit 9.10
DOI:  10.1002/0471142735.im0910s109
Online Posting Date:  April, 2015
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Proteasome inhibitors are indispensable research tools in immunology and cell biology. With numerous proteasome inhibitors available commercially, choosing the appropriate compound for a biological experiment may be challenging, especially for a novice. This unit provides an overview of the proteasome inhibitors commonly used in research. It discusses how to select an appropriate highly specific inhibitor, its concentration, and length of exposure for mammalian cell culture experiments. In addition, assays that can be used to confirm proteasome inhibition are discussed. © 2015 by John Wiley & Sons, Inc.

Keywords: proteosome inhibitors; peptide aldehydes; boronates; peptide epoxyketones

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Table of Contents

  • Introduction
  • Specificity of Proteasome Inhibitors Depends on Their Chemical Structure
  • Choosing the Correct Concentration of a Proteasome Inhibitor
  • Measurement of Proteasome Inhibition
  • Proteasome‐Inhibitor Induced Apoptosis
  • Interpretation of Results of Proteasome Inhibitor Experiments
  • Summary
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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