Efficient In Vivo Delivery of Plasmids and Oligonucleotides Using Hemagglutinating Virus of Japan Envelope (HVJ‐E) Vector in Immunological Disease Models

Atsushi Kitani1, Stefan Fichtner‐Feigl2

1 National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, 2 University of Regensburg, Regensburg, Germany
Publication Name:  Current Protocols in Immunology
Unit Number:  Unit 10.17E
DOI:  10.1002/0471142735.im1017es91
Online Posting Date:  November, 2010
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

This unit describes a method for in vivo delivery of oligonucleotides or plasmids using the hemagglutinating virus of Japan envelope (HVJ‐E), an inactivated Sendai virus particle, as a delivery system. Viral transfection methods generally show a higher transfection efficiency than nonviral methods for the delivery of genes to cells. However, in using these methods one must bear in mind that the introduction of a virus particle into a host carries a risk for leukemia induction and for creation of disturbances in immune function due to cytotoxicity. Curr. Protoc. Immunol. 91:10.17E.1‐10.17E.9. © 2010 by John Wiley & Sons, Inc.

Keywords: hemagglutinating virus of Japan; Sendai virus; plasmid; siRNA; decoy oligo; gene therapy; in vivo

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • Introduction
  • Basic Protocol 1: Plasmid Packaging into the HVJ‐E Particle
  • Alternate Protocol 1: Oligonucleotides Delivery with HVJ‐E Particle
  • Reagents and Solutions
  • Commentary
  • Literature Cited
  • Figures
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

Basic Protocol 1: Plasmid Packaging into the HVJ‐E Particle

  Materials
  • HVJ envelope (see recipe), ice‐cold
  • 1 mg/ml protamine sulfate/PBS (see recipe), ice‐cold
  • Plasmid
  • Dulbecco's phosphate‐buffered saline (D‐PBS)
  • 3% Triton X‐100 (see recipe), ice‐cold
  • 1.7‐ml microcentrifuge tubes
  • 1‐ml syringes equipped with 26‐G needles
NOTE: All equivalent materials described above, other than the plasmid, are contained in the GenomONE Neo EX HVJ Envelope Transfection Kit supplied from Ishihara Sangyo Kaisha or Cosmo Bio (http://www.cosmobio.co.jp/export_e/products/cells/products_ISK_20070531.asp).

Alternate Protocol 1: Oligonucleotides Delivery with HVJ‐E Particle

  • Oligonucleotides (siSTABLE siRNA from Dharmacon/Thermoscientific or phosphothioate DNA) dissolved at the concentration of 10 mg/ml in nuclease free, ice‐cold PBS
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

Literature Cited
   Cavazzana‐Calvo, M., Thrasher, A., and Mavillo, F. 2004. The future of gene therapy. Nature 427:779‐781.
   Fichtner‐Feigl, S., Fuss, I.J., Preiss, J.C., Strober, W., and Kitani, A. 2005. Treatment of murine Th1‐ and Th2‐mediated inflammatory bowel disease with NF‐kappa B decoy oligonucleotides. J. Clin. Invest. 115:3057‐3071.
   Fichtner‐Feigl, S., Strober, W., Kawakami, K., Puri, R.K., and Kitani, A. 2006. IL‐13 signaling through the IL‐13alpha2 receptor is involved in induction of TGF‐beta1 production and fibrosis. Nat. Med. 12:99‐106.
   Fichtner‐Feigl, S., Fuss, I.J., Young, C.A., Watanabe, T., Geissler, E.K., Schlitt, H.J., Kitani, A., and Strober, W. 2007. Induction of IL‐13 triggers TGF‐beta1‐dependent tissue fibrosis in chronic 2,4,6‐trinitrobenzene sulfonic acid colitis. J. Immunol. 178:5859‐5870.
   Fujihara, A., Kurooka, M., Miki, T., and Kaneda, Y. 2008. Intratumoral injection of inactivated Sendai virus particles elicits strong antitumor activity by enhancing local CXCL10 expression and systemic NK cell activation. Cancer Immunol. Immunother. 57:73‐84.
   Kaneda, Y. 2008. Applications of Hemagglutinating Virus of Japan in therapeutic delivery systems. Expert Opin. Drug Deliv. 5:221‐233.
   Kaneda, Y., Uchida, T., Kim, J., Ishiura, M., and Okada, Y. 1987. The improved efficient method for introducing macromolecules into cells using HVJ (Sendai virus) liposomes with gangliosides. Exp. Cell Res. 173:56‐69.
   Kaneda, Y., Saeki, Y., and Morishita, R. 1999. Gene therapy using HVJ‐liposomes: The best of both worlds? Mol. Med. Today 5:298‐303.
   Kaneda, Y., Nakajima, T., Nishikawa, T., Yamamoto, S., Ikegami, H., Suzuki, N., Nakamura, H., Morishita, R., and Kotani, H. 2002. Hemagglutinating virus of Japan (HVJ) envelope vector as a versatile gene delivery system. Mol. Ther. 6:219‐226.
   Kaneda, Y., Yamamoto, S., and Nakajima, T. 2005. Development of HVJ envelope vector and its application to gene therapy. Adv. Genet. 53PA:307‐332.
   Kawaguchi, Y., Miyamoto, Y., Inoue, T., and Kaneda, Y. 2008. Efficient eradication of hormone‐resistant human prostate cancers by inactivated Sendai virus particle. Int. J. Cancer 124:2478‐2487.
   Kimura, T., Nakamura, H., Koyama, S., Ogita, K., Tabata, C., Tsutsui, T., Shimoya, K., Koyama, M., Kaneda, Y., and Murata, Y. 2005. In vivo gene transfer into the mouse uterus: A powerful tool for investigating implantation physiology. J. Reprod. Immunol. 67:13‐20.
   Kondo, Y., Fushikida, K., Fujieda, T., Sakai, K., Miyata, K., Kato, F., and Kato, M. 2008. Efficient delivery of antibody into living cells using a novel HVJ envelope vector system. J. Immunol. Methods 332:10‐17.
   Kurooka, M. and Kaneda, Y. 2007. Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells. Cancer Res. 67:227‐236.
   Okada, Y. 1993. Sendai virus‐induced cell fusion. Methods Enzymol. 221:18‐41.
   Slobod, K.S., Shenep, J.L., Luján‐Zilbermann, J., Allison, K., Brown, B., Scroggs, R.A., Portner, A., Coleclough, C., and Hurwitz, J.L. 2004. Safety and immunogenicity of intranasal murine parainfluenza virus type 1 (Sendai virus) in healthy human adults. Vaccine 22:3182‐3186.
   Yang, Z., Fuss, I.J., Watanabe, T., Asano, N., Davey, M.P., Rosenbaum, J.T., Strober, W., and Kitani, A. 2007. NOD2 transgenic mice exhibit enhanced MDP‐mediated down‐regulation of TLR2 responses and resistance to colitis induction. Gastroenterology 133:1510‐1521.
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library