Immunophenotyping of T Cell Subpopulations in HIV Disease

Pratip K. Chattopadhyay1, Mario Roederer1

1 National Institutes of Health, Bethesda, Maryland
Publication Name:  Current Protocols in Immunology
Unit Number:  Unit 12.12
DOI:  10.1002/0471142735.im1212s65
Online Posting Date:  March, 2005
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This unit illustrates the challenges associated with measurements of T cell subpopulations, and describes how those challenges can be overcome by using polychromatic (5+ color) approaches to flow cytometry. Such approaches are immensely powerful and directly applicable to studies of T cell biology in HIV disease; however, their development requires careful consideration of the antibodies and fluorochromes employed, as discussed here. Furthermore, factors generally known to influence T cell counts can also govern the success or failure of polychromatic experiments; therefore, these factors are reviewed and provide recommendations for minimizing their influence on polychromatic measurements of T cell subsets.

Keywords: Polychromatic flow cytometry; T cell subsets; immunophenotyping; CD4 count

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Table of Contents

  • The Role of CD4+ and CD8+ Subpopulations in T Cell Biology
  • Challenges Associated with Identification of T Cell Subpopulations: The Need for Polychromatic (5+ Color) Immunophenotyping
  • Strategies for Developing Polychromatic Flow Cytometry Technology
  • Application of Polychromatic Flow Cytometry
  • Lessons Learned from Enumeration of CD4+ T Cells
  • Conclusions
  • Acknowledgements
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

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