Affinity‐Based Biosensors for Biomolecular Interaction Analysis

Kerstin Gunnarsson1

1 BIACORE AB, Uppsala
Publication Name:  Current Protocols in Immunology
Unit Number:  Unit 18.6
DOI:  10.1002/0471142735.im1806s33
Online Posting Date:  May, 2001
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Abstract

This overview presents an introduction to affinity‐based biosensors, most notably the BIACORE. These biosensors detect molecular interactions by immobilizing one of the interactants (the ligand or target molecule) on the surface of a sensor chip. A solution containing the other interactant is then applied to the chip. Binding of analyte to the surface results in a response proportional to the mass bound. A discussion of sensor chips and practical applications is also provided.

     
 
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Table of Contents

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Materials

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Literature Cited

Literature Cited
   Bartley, T.D., Hunt, R.W., Welcher, A.A., Boyle, W.J., Parker, V.P., Lindberg, R.A., Lu, H.S., Colombero, A.M., Elliott, R.L., Guthrie, B.A., Holst, P.L., Skrine, J.D., Toso, R.J., Zhang, M., Fernandez, E., Trail, G., Varnum, B., Yarden, Y., Hunter, T., and Fox, G.M. 1994. B61 is a ligand for the ECK receptor protein‐tyrosine kinase. Nature 368:558‐560.
   Johne, B., Gadnell, M., and Hansen, K. 1993. Epitope mapping and binding kinetics of monoclonal antibodies studied by real time biospecific interaction analysis using surface plasmon resonance. J. Immunol. Methods 160:191‐198.
   Jönsson, U. and Malmqvist, M. 1992. Real time biospecific interaction analysis: The integration of surface plasmon resonance detection, general biospecific interface chemistry and microfluidics into one analytical system. Adv. Biosens. 2:291‐336.
   Masson, L., Lu, Y.J., Mazza, A., Brousseaur, R., and Adang, M.J. 1995. Kinetics of Bacillus thuringiensis toxin binding with brush border membrane vesicles from susceptible and resistant larvae of Plutella xylostella. J. Biol. Chem. 270:11887‐11896.
   Schuster, S.C., Swanson, R.V., Alex, L.A., Bourret, R., and Simon, M.I. 1993. Assembly and function of a quaternary signal transduction complex monitored by surface plasmon resonance. Nature 365:343‐347.
Internet Resources
   http://www.biacore.com
  BIACORE Web site. Contains list of references.
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