Fluorescence Resonance Energy Transfer: Techniques for Measuring Molecular Conformation and Molecular Proximity

Michael Edidin1

1 Johns Hopkins University, Baltimore, Maryland
Publication Name:  Current Protocols in Immunology
Unit Number:  Unit 18.10
DOI:  10.1002/0471142735.im1810s52
Online Posting Date:  February, 2003
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Abstract

This overview unit focuses on the basics of fluorescence and of the FRET phenomenon, and on methods for detecting FRET and data interpretation. FRET is very versatile and there are more application of these basics than can be covered in a single overview. However, some examples are given of applications of various FRET techniques.

     
 
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Table of Contents

  • Basics of Fluorescence and FRET Mechanisms
  • Practical FRET Microscopy
  • Donor Dequenching After Acceptor Photobleaching
  • Donor Fluorescence Lifetime Imaging
  • Frequency Domain Lifetime Imaging
  • Analysis of FRET Data
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
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