Commonly Used Detergents

John E. Coligan1

1 National Institute of Allergy and Infectious Diseases, Bethesda, Maryland
Publication Name:  Current Protocols in Immunology
Unit Number:  Appendix 1D
DOI:  10.1002/0471142735.ima01ds17
Online Posting Date:  May, 2001
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Abstract

This appendix lists reference Epstein‐Barr virus (EBV)‐transformed B lymphoblastoid cell lines (B‐LCL) and the HLA types expressed by these cell lines. These B‐LCL are useful for studies of cellular immunity (e.g., restriction of antigen‐specific responses), biochemical characterization of histocompatibility molecules, and as controls for DNA‐based typing methods.

     
 
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Table of Contents

  • Types of Detergents
  • Choice of Detergents
  • Tables
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
   Furth, A.J., Bolton, H., Potter, J., and Priddle, J.D. 1984. Separating detergents from proteins. Methods Enzymol. 104:318‐328.
   Harlow, E. and Lane, D. 1988. Detergents. In Antibodies: A Laboratory Manual, pp. 687‐689. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Helenius, A., McCaslin, D.R., Fries, E., and Tanford, C. 1979. Properties of detergents. Methods Enzymol. 56:734‐749.
   Hjelmand, L.M. 1979. Removal of detergents from membrane proteins. Methods Enzymol. 182:277‐282.
   Jones, O.T., Earnest, J.P., and McNamee, M.G. 1987. Solubilization and reconstitution of membrane proteins. In Biological Membranes: A Practical Approach (J. Findlay and W.H. Evans, eds.), pp.142‐143. IRL Press, Oxford.
   Smith, J.A., Colorimetric methods. 1987. In Current Protocols in Molecular Biology (F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl eds.), pp. 10.1.1‐10.1.3. Greene Publishing and Wiley‐Interscience, New York.
Key References
   Harlow and Lane, 1988. See above.
   Hjelmeland, L.M. and Chrambach, A. 1984. Solubilization of functional membrane proteins. Methods Enzymol. 182:305‐318.
   Johnstone, A. and Thorpe, R. 1982. Isolation and fractionation of lymphocytes. In Immunochemistry in Practice, pp.94‐101. Blackwell Scientific, Oxford.
   Neugebaur, J.M. 1990. Detergents: An overview. Methods Enzymol. 182:239‐282.
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