HLA Type of EBV‐Transformed Human B Cell Lines

Carolyn Katovich Hurley1, Armead H. Johnson1

1 Georgetown University School of Medicine, Washington, D.C
Publication Name:  Current Protocols in Immunology
Unit Number:  Appendix 1G
DOI:  10.1002/0471142735.ima01gs30
Online Posting Date:  May, 2001
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

This appendix lists reference Epstein‐Barr virus (EBV)‐transformed B lymphoblastoid cell lines (B‐LCL) and the HLA types expressed by these cell lines. These B‐LCL are useful for studies of cellular immunity (e.g., restriction of antigen‐specific responses), biochemical characterization of histocompatibility molecules, and as controls for DNA‐based typing methods.

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • HLAType of EBV‐Transformed Human B Cell Lines
  • Literature Cited
  • Figures
  • Tables
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

Literature Cited
   Bodmer, J.G., Marsh, S.G.E., and Albert, E. 1990. Nomenclature for factors of the HLA system, 1989. Immunol. Today 11:3‐10.
   Bodmer, J.G., Marsh, S.G.E., Albert, E.D., Bodmer, W.F., Dupont, B., Erlich, H.A., Mach, B., Mayr, W.R., Parham, P., Sasazuki, T., Schreuder, G.M.T., Strominger, J.L., Svejgaard, A., and Terasaki, P.I. 1991. Nomenclature for factors of the HLA system, 1990. Hum. Immunol. 31:186‐194.
   Bodmer, J.G., Marsh, S.G.E., Albert, E.D., Bodmer, W.F., Dupont, B., Erlich, H.A., Mach, B., Mayr, W.R., Parham, P., Sasazuki, T., Schreuder, G.M.T., Strominger, J.L., Svejgaard, A., and Terasaki, P.I. 1992. Nomenclature for factors of the HLA system, 1991. Hum. Immunol. 34:4‐18.
   European Collection for Biomedical Research Catalogue. 1992. Eur. J. Immunogenet. 19:181‐251.
   Fernandez‐Vina, M.A., Gao, X., Moraes, M.E., Moraes, J.R., Salatiel, I., Miller, S., Tsai, J., Sun, Y., An, J., Layrisse, Z., Gazit, E., Brautbar, C., and Stastny, P. 1991. Alleles at four HLA class II loci determined by oligonucleotide hybridization and their associations in five ethnic groups. Immunogenetics 34:299‐312.
   Kimura, A., Dong, R.‐P., Harada, H., and Sasazuki, T. 1992. DNA typing of HLA Class II genes in B‐lymphoblastoid cell lines homozygous for HLA. Tissue Antigens 40:5‐12.
   Molkentin, J., Gorski, J., and Baxter‐Lowe, L.A. 1991. Detection of 14 HLA‐DQB1 alleles by oligotyping. Hum. Immunol. 31:114‐122.
   Schreuder, G.M.T., Doxiadis, I., Parlevliet, J., and Grosse‐Wilde, H. 1984. HLA‐DR, DQ, LB, and Ta10 specificities of Ninth Workshop Homozygous Typing Cells. In Histocompatibility Testing (E.D. Albert, M.P. Baur, and W.R. Mayr, eds.) pp. 243‐248, Springer‐Verlag, N.Y.
   Sutton, V.R., Kienzle, B.K., and Knowles, R.W. 1989. An altered splice site is found in the DRB4 gene that is not expressed in HLA‐DR7, Dw11 individuals. Immunogenetics 29:317‐322.
   Vaughan, R.W., Lanchbury, J.S.S., Marsh, S.G.E., Hall, M.A., Bodmer, J.G., and Welsh, K.I. 1990. The application of oligonucleotide probes to HLA class II typing of the DRB sub‐region. Tissue Antigens 36:149‐155.
   Yang, S.Y., Milford, E., Hammerling, U., and Dupont, B. 1989. Description of the reference panel of B‐lymphoblastoid cell lines for factors of the HLA system: The B‐cell line panel designed for the Tenth International Histocompatibility Workshop. In Immunobiology of HLA, Vol. I: Histocompatibility Testing (B. Dupont, ed.) pp.11‐19, Springer‐Verlag, N.Y.
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library