Biosafety: Guidelines for Working with Pathogenic and Infectious Microorganisms

Richard Coico1, George Lunn2

1 Weill Medical College of Cornell University, New York, New York, 2 null, Baltimore, Maryland
Publication Name:  Current Protocols in Immunology
Unit Number:  Appendix 1V
DOI:  10.1002/0471142735.ima01vs69
Online Posting Date:  November, 2005
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Abstract

This unit, in conjunction with local and national guidelines and regulations, provides the basic biosafety information needed to perform the procedures detailed in this manual. Topics discussed include routine precautions when working with biohazards, disinfectants, disposal of biohazards, biosafety levels (as established by the U.S. National Institutes of Health, and the Centers for Disease Control and Prevention), animal facilities, and clinical laboratories. In addition, resources for more information are provided in the Literature Cited and Key References sections and in URLs given text and within the Internet Resources section.

Keywords: biosafety; biosafety levels; animal biosafety levels; biosafety guidelines; biosafety regulations; biohazards; pathogens

     
 
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Table of Contents

  • General Biosafety Guidelines
  • Biosafety Levels
  • Animal Facilities
  • Clinical Laboratories
  • Literature Cited
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

   Morbidity and Mortality Weekly Report (MMWR), Centers for Disease Control. 1988. Update: Universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus and other bloodborne pathogens in healthcare settings. MMWR 37:377‐388.
   National Committee for Clinical Laboratory Standards (NCCLS). 1997. Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, Body Fluids, and Tissue. Approved Guideline, Dec. 1977, NCCLS Doc. M29‐A. NCCLS, Wayne, Penn.
   Occupational Safety and Health Administration (OSHA), U.S. Department of Labor. 1991. Occupational exposure to bloodborne pathogens, final rule. In Federal Register Volume 56, pp. 64175‐64182. U.S. Government Printing Office, Washington D.C.
   Richmond, J.Y. 1994. HIV Biosafety: Guidelines and Regulations. In AIDS Testing, Edition 2 (G. Schochetman and J. R. George, eds.) pp. 346‐360. Springer‐Verlag, New York.
Key References
   U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention (CDC) and National Institutes of Health (NIH). 1999. Biosafety in Microbiological and Biomedical Laboratories (BMBL), 4th Ed. 1999. (J.Y. Richmond and R.W. McKenney, eds.) U.S. Government Printing Office, Washington D.C. (GPO S/N 017‐040‐00547‐4).
   Fuscaldo, A.A., Erlick, B.J., and Hindman, B. (eds.). 1980. Laboratory Safety, Theory and Practice. Academic Press, San Diego.
Internet Resources
   http://www.OSHA.gov
  OSHA web site
   http://www.cdc.gov
  The Centers for Disease Control and Prevention website
   http://tis.eh.doe.gov/docs/osh_tr/ch5.html
  DOE OSH technical reference chapter on personal protective equipment
   http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm
  Online version of the Biosafety in Microbiological and Biomedical Laboratories (BMBL), 4th Ed. See for print version information.
   http://bmbl.od.nih.gov
  BMBL version with longer than average web pages and minimal graphics for ease of printing
   http://www.cdc.gov/od/ohs/pdffiles/4th%20BMBL.pdf
  PDF version of the BMBL. See above for other versions.
   http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4s6.htm
  Provides information on recommended biosafety levels for infectious agents and infected animals.
   http://www.absa.org/resguides.html
  The American Biological Safety Association (ABSA) Biosafety Guidelines website provides links to international biosafety guidelines and websites.
  http://www.ebsa.be
  In addition to being a source of information in and of itself, the European Biosafety Association homepage is also host to the International Biosafety Working Group (IBWG), a compendium of international regulations and guidelines with descriptions and URLs. Access by clicking International Biosafety from the menu bar on the top of the screen.
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