Common Media, Buffers, and Stock Solutions


Publication Name:  Current Protocols in Immunology
Unit Number:  Appendix 2A
DOI:  10.1002/0471142735.ima02as34
Online Posting Date:  May, 2001
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Abstract

This section describes the preparation of media, buffers, and reagents used in this manual.

    Culture Media

  • Complete Click's medium

  • Complete DMEM medium

  • Complete IMDM medium

  • Complete RPMI medium

    Buffers and Stock Solutions

  • Acids, concentrated stock solutions

  • Alsever's solution

  • Ammonium acetate, 10 M

  • Carbonate buffer

  • Denhardt solution, 100×

  • DTT (dithiothreitol), 1 M

  • EDTA (ethylenediaminetetraacetic acid), 0.5 M (pH 8.0)

  • HBSS (Hanks' balanced salt solution)

  • HCl, 1 M

  • 2‐ME (2‐mercaptoethanol), 50 mM

  • MgCl2, 1 M

  • NaCl, 5 M

  • NaOH, 10 M

  • PBS (phosphate‐buffered saline)

  • Potassium phosphate buffer, 0.1 M

  • SDS, 20% (w/v)

  • Sodium acetate, 3 M

  • Sodium acetate buffer, pH 4.0 to 5.0

  • Sodium phosphate buffer, 0.1 M

  • TBS (Tris‐buffered saline)

  • TE (Tris/EDTA) buffer

  • Tris⋅Cl, 1 M

     
 
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Table of Contents

  • Culture Media
  • Buffers and Stock Solutions
  • Literature Cited
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Chemical Rubber Company. 1975. CRC Handbook of Biochemistry and Molecular Biology, Physical and Chemical Data 3d ed., Vol. 1. CRC Press, Boca Raton, Fla.
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