Monitoring Cell Growth

Warren Strober1

1 National Institute of Allergy and Infectious Diseases, Bethesda, Maryland
Publication Name:  Current Protocols in Immunology
Unit Number:  Appendix 3A
DOI:  10.1002/0471142735.ima03as21
Online Posting Date:  May, 2001
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Abstract

This appendix provides two protocols for monitoring cell growth. Counting cells using a hemacytometer is tedious but it allows one to effectively distinguish live cells from dead cells (using Trypan Blue exclusion). In addition, this procedure is less subject to errors due to cell clumping or heterogeneity of cell size. The use of an electronic cell counter is quicker and easier than counting cells using a hemacytometer. However, an electronic cell counter as currently constructed does not distinguish live from dead cells in a reliable fashion and is subject to error due to the presence of cell clumps. Overall, the electronic cell counter is best reserved for repetitive and rapid counting of fresh peripheral blood cells and should be used with caution when counting cell populations derived from tissues.

     
 
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Table of Contents

  • Basic Protocol 1: Counting Cells Using a Hemacytometer
  • Basic Protocol 2: Counting Cells Using a Coulter Counter
  • Commentary
     
 
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Materials

Basic Protocol 1: Counting Cells Using a Hemacytometer

  Materials
  • Hemacytometer with coverslip (Thomas Scientific)
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Figures

Literature Cited

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