Silanizing Glassware

Brian Seed1

1 Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts
Publication Name:  Current Protocols in Immunology
Unit Number:  Appendix 3K
DOI:  10.1002/0471142735.ima03ks21
Online Posting Date:  May, 2001
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Abstract

Glassware is silanized (siliconized) to prevent adsorption of solute to glass surfaces or to increase its hydrophobicity. This is particularly important with low concentrations of particularly “sticky“ solutes such as single‐stranded nucleic acids or proteins. This appendix provides a protocol for treating glassware.

     
 
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Table of Contents

  • Commentary
     
 
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Materials

Basic Protocol 1:

  Materials
  • Chlorotrimethylsilane (Sigma) or dichlorodimethylsilane (Sigma)
  • Vacuum pump
  • Desiccator, equipped with a valve
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Figures

Videos

Literature Cited

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