Preparing Frozen Bacterial Stocks

Sherie L. Morrison1

1 University of California Los Angeles, Los Angeles, California
Publication Name:  Current Protocols in Immunology
Unit Number:  Appendix 3M
DOI:  10.1002/0471142735.ima03ms21
Online Posting Date:  May, 2001
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Abstract

After bacteria containing plasmids of interest have been produced, it is frequently convenient to store them for later use. This appendix provides instruction for storing bacteria in such a way that they typically remain viable for years.

     
 
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Table of Contents

  • Reagents and Solutions
  • Commentary
     
 
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Materials

Basic Protocol 1:

  Materials
  • recipeLB medium with and without appropriate antibiotic (unit 10.18)
  • Bacteria
  • recipeLB medium with glycerol (see recipe)
  • 15‐ml polystyrene tube, sterile
  • 1.5‐ml microcentrifuge tube, sterile
  • Inoculating loop, sterile
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Figures

Videos

Literature Cited

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