Growth in Liquid Media

Karen Elbing1, Roger Brent2

1 Clark & Elbing LLP, Boston, Massachusetts, 2 The Molecular Sciences Institute, Berkeley, California
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 1.2
DOI:  10.1002/0471142727.mb0102s59
Online Posting Date:  August, 2002
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Abstract

The procedure for inoculating overnight (starter) cultures of E. coli from a single colony is described along with considerations for growing larger cultures. Also included are two methods for monitoring cell growth using a spectrophotometer or a hemacytometer.

     
 
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Table of Contents

  • Basic Protocol 1: Growing an Overnight Culture
  • Basic Protocol 2: Growing Larger Cultures
  • Basic Protocol 3: Monitoring Growth
  • Figures
     
 
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Materials

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Figures

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Literature Cited

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