Growth on Solid Media

Karen Elbing1, Roger Brent2

1 Clark & Elbing LLP, Boston, Massachusetts, 2 The Molecular Sciences Institute, Berkeley, California
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 1.3
DOI:  10.1002/0471142727.mb0103s59
Online Posting Date:  August, 2002
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Abstract

Detailed protocols are provided for titering and isolating bacterial colonies by serial dilutions, or alternatively by streaking or spreading a plate. Support protocols describe replica plating as well as methods for storing strains as agar stabs or frozen glycerol stocks.

     
 
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Table of Contents

  • Basic Protocol 1: Titering and Isolating Bacterial Colonies by Serial Dilutions
  • Basic Protocol 2: Isolating Single Colonies by Streaking a Plate
  • Basic Protocol 3: Isolating Single Colonies by Spreading a Plate
  • Support Protocol 1: Replica Plating
  • Support Protocol 2: Strain Storage and Revival
  • Reagents and Solutions
  • Figures
     
 
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Materials

Basic Protocol 1: Titering and Isolating Bacterial Colonies by Serial Dilutions

  Materials
  • recipeLB medium (unit 1.1)
  • recipeLB plates (unit 1.1)
  • Sterile 16‐ or 18‐mm‐diameter culture tubes
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Figures

Videos

Literature Cited

Literature Cited
   Adams, J.N. 1965. Automotive pistons for use as bases in velveteen replication. J. Bacteriol. 89:1627.
Key Reference
   Lederberg, J. and Tatum, E.L. 1953. Novel genotypes in mixed cultures of biochemical mutants of bacteria. Cold Spring Harbor Symp. Quant. Biol. 18:75.
  A primary reference in this field of research.
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