Selected Topics from Classical Bacterial Genetics

Elisabeth A. Raleigh1, Karen Elbing2, Roger Brent3

1 New England Biolabs, Beverly, Massachusetts, 2 Clark & Elbing LLP, Boston, Massachusetts, 3 The Molecular Sciences Institute, Berkeley, California
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 1.4
DOI:  10.1002/0471142727.mb0104s59
Online Posting Date:  August, 2002
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Current cloning technology exploits many facts learned from classical bacterial genetics. This unit covers those that are critical to understanding the techniques described in this book. Topics include antibiotics, the LAC operon, the F factor, nonsense suppressors, genetic markers, genotype and phenotype, DNA restriction, modification and methylation and recombination.

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Table of Contents

  • Antibiotics
  • The Lac Operon
  • The F Factor
  • Nonsense Suppressors
  • Genetic Markers
  • Genotype and Phenotype
  • DNA Restriction, Modification, and Methylation
  • Recombination and Its Effects on Cloned DNA Inserts
  • Effects of Recombination‐Defective Strains on Vectors
  • Figures
  • Tables
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Literature Cited

Literature Cited
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Key References
   Beckwith, J. and Zipser, D. eds. 1970. The Lactose Operon. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y
  An introduction to early work with the lactose operon.
   Miller, 1972. See above
  Contains excellent introductions to experimental techniques for working with E. coli and λ‐derived phages.
   Miller, J., ed. 1981. The Operon. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y
  An updated and thorough introduction to regulatory mechanisms, starting with the lactose operon.
   Neidhardt, F.C., Ingraham, J.L., Low, K.B., Magasanik, B., Schaechter, M., and Umbarger, H.E., eds. 1996. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. American Society for Microbiology, Washington, D.C
  Encyclopedic coverage of the biology of these useful bacteria.
   Ullman, A. and Perrin, D. 1970. Complementation in β‐galactosidase. In The Lactose Operon (J. Beckwith and D. Zipser, eds.) pp. 143‐172. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y
  Definitive review of alpha‐complementation.
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