Introduction to Vectors Derived from Filamentous Phages

David Greenstein1, Roger Brent1

1 Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 1.14
DOI:  10.1002/0471142727.mb0114s13
Online Posting Date:  May, 2001
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Abstract

Many vectors in current use are derived from filamentous phages. These vectors are used because DNA inserted into them can be recovered in two forms: double‐stranded circles and single‐stranded circles. This overview unit describes the lifecycle of filamentous phages along with the development and use of filamentous phage vectors.

     
 
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Table of Contents

  • Development and Use of Filamentous Phage Vectors
  • Lifecycle of Filamentous Phages
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
   Beck, E. and Zink, B. 1981. Nucleotide sequence and genome organization of filamentous bacteriophage f1 and bacteriophage fd. Gene. 16:35‐38.
   Brewer, B.J. 1988. When polymerases collide: Replication and the transcriptional organization of the E. coli chromosome. Cell. 53:679‐686.
   Dente, L., Cesareni, G., and Cortese, R. 1983. pEMBL: A new family of single‐stranded plasmids. Nucl. Acids Res. 11:1645‐1655.
   Greenstein, D. and Horiuchi, K. 1988. Integration host factor interacts with the DNA replication enhancer of filamentous phage f1. Proc. Natl. Acad. Sci. U.S.A. 85:6262‐6266.
   Messing, J. 1983. New M13 vectors for cloning. Methods Enzymol. 101:20‐78.
   Messing, J., Gronenborn, B., Muller‐Hill, B., and Hofschneider, P.H. 1977. Single‐strand filamentous DNA phage as a carrier for in vitro recombined DNA. Proc. Natl. Acad. Sci. U.S.A. 74:3642‐3646.
   Rasched, I. and Oberer, E. 1986. Ff coliphages: Structural and functional relationships. Microbiol. Rev. 50:401‐427.
   Yanisch‐Perron, C., Vieira, J., and Messing, J. 1985. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33:103‐119.
   Zinder, N.D. and Boeke, J.D. 1982. The filamentous phage as vector for recombinant DNA: A review. Gene. 19:1‐10.
   Zinder, N.D. and Horiuchi, K. 1985. Multiregulatory element of filamentous bacteriophages. Microbiol. Rev. 9:101‐106.
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