Ribonucleases

Nicole M. Nichols1, Dongxian Yue1

1 New England Biolabs, Ipswich, Massachusetts
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 3.13
DOI:  10.1002/0471142727.mb0313s84
Online Posting Date:  October, 2008
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Abstract

Ribonucleases (RNases) with different sequence or structural specificities are used for a variety of analytical purposes, including RNA sequencing, mapping, and quantitation. The development of RNase protection assays, structural determination assays, and the production of small interfering RNAs (siRNA) employed in RNA interference (RNAi) experiments has depended on the unique substrate specificities of commercially available RNases, including RNases A, I, T1, V1, HI, III, and Dicer. One very common application for high purity RNase A is also presented in this unit and involves hydrolyzing RNA that contaminates DNA preparations. RNase HII and the placental RNase inhibitor are also discussed. Curr. Protoc. Mol. Biol. 84:3.13.1‐3.13.8. © 2008 by John Wiley & Sons, Inc.

Keywords: RNase; RNase protection assay; RNA footprinting; RNAi; siRNA

     
 
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Table of Contents

  • Introduction
  • Enzyme: Ribonuclease A
  • Enzyme: Ribonuclease I
  • Enzyme: Ribonuclease T1
  • Enzyme: Ribonuclease HI
  • Enzyme: Ribonuclease HII
  • Enzyme: Ribonuclease V1
  • Enzyme: Ribonuclease III
  • Enzyme: Dicer
  • Ribonuclease Inhibitor
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Literature Cited

Literature Cited
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