Genomic DNA Libraries

David D. Moore1

1 Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 5.1
DOI:  10.1002/0471142727.mb0501s00
Online Posting Date:  May, 2001
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

Genomic DNA libraries are almost always screened by hybridization using a radioactive nucleic acid probe. Since this approach is essentially independent of a particular vector or type of target DNA, the main problem faced when considering creation of a genomic DNA library is simply generating a large enough number of recombinant DNA clones. The basic strategies used to address this problem have included both minimizing the number of clones necessary by incorporating large fragments of genomic DNA, and maximizing cloning efficiency by using vectors based on bacteriophage l. This unit discusses the appropriate numerical considerations for both ordinary genomic DNA libraries and subgenomic DNA libraries, and then describes a limited number of appropriate vectors.

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • Section I: Overview of Recombinant DNA Libraries
  • Representation and Randomness
  • Subgenomic DNA Libraries
  • Vectors for Genomic DNA Libraries
  • Literature Cited
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

Literature Cited
   Clark, L. and Carbon, J. 1976. A colony bank containing synthetic ColE1 hybrids representative of the entire E. coli genome. Cell 9:91‐99.
   Ish‐Horowitz, D. and Burke, J.F. 1981. Rapid and efficient cosmid vector cloning. Nucl. Acids Res. 9:2989‐2999.
   Seed, B., Parker, R.C., and Davidson, N. 1982. Representation of DNA sequences in recombinant DNA libraries prepared by restriction enzyme partial digestion. Gene 19:201‐209.
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library