Overview of DNA Sequencing Strategies

Jay A. Shendure1, Gregory J. Porreca2, George M. Church3, Andrew F. Gardner4, Cynthia L. Hendrickson5, Jan Kieleczawa6, Barton E. Slatko4

1 Department of Genome Sciences, University of Washington, Seattle, Washington, 2 Good Start Genetics, Inc., Cambridge, Massachusetts, 3 Department of Genetics, Harvard Medical School, Boston, Massachusetts, 4 New England Biolabs, Ipswich, Massachusetts, 5 HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, 6 Wyzer Biosciences, Cambridge, Massachusetts
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 7.1
DOI:  10.1002/0471142727.mb0701s96
Online Posting Date:  October, 2011
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Abstract

Efficient and cost‐effective DNA sequencing technologies are critical to the progress of molecular biology. This overview of DNA sequencing strategies provides a high‐level review of seven distinct approaches to DNA sequencing: (a) dideoxy sequencing; (b) solid phase sequencing; (c) sequencing‐by‐hybridization; (d) mass spectrometry; (e) cyclic array sequencing; (f) microelectrophoresis; and (g) nanopore sequencing. Other platforms currently in development are also briefly described. The primary focus here is on Sanger dideoxy sequencing, which has been the dominant technology since 1977, and on cyclic array strategies, for which several competitive implementations have been developed since 2005. Because the field of DNA sequencing is changing rapidly, this unit represents a snapshot as of September, 2011. Curr. Protoc. Mol. Biol. 96:7.1.1‐7.1.23. © 2011 by John Wiley & Sons, Inc.

Keywords: DNA sequencing; next‐generation sequencing; polony; cyclic array sequencing; genomics; sequencing by ligation; nanopore sequencing

     
 
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Table of Contents

  • Introduction
  • DNA Sequencing Strategies
  • Choosing a Sequencing Strategy
  • Platforms
  • Handling NextGen Sequence Data
  • Additional Technologies on the Horizon
  • Literature Cited
  • Figures
     
 
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Materials

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Literature Cited

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