Preparation of Yeast Media

Douglas A. Treco1, Victoria Lundblad2

1 Massachusetts General Hospital, Boston, Massachusetts, 2 Baylor College of Medicine, Houston, Texas
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 13.1
DOI:  10.1002/0471142727.mb1301s23
Online Posting Date:  May, 2001
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Abstract

Preparation of sterile media of consistently high quality is essential for the genetic manipulation of yeast. Recipes for media needed in the protocols in this chapter are provided in this unit. Specific suppliers are recommended for specific ingredients.

     
 
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Table of Contents

  • Section I: Basic Techniques of Yeast Genetics
  • Liquid Media
  • Solid Media
  • Strain Storage and Revival
  • Basic Protocol 1: Preparation and Inoculation of Frozen Stocks
  • Alternate Protocol 1: Preparation and Inoculation of Slants
  • Basic Protocol 2: Mailing and Reviving Strains
  • Literature Cited
  • Tables
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
   Boeke, J.D., LaCroute, F., and Fink, G.R. 1984. A positive selection for mutants lacking orotidine‐5′‐phosphate decarboxylase activity in yeast: 5‐fluoroorotic acid resistance. Mol. Gen. Genet. 197:345‐346.
   Brown, P.A. and Szostak, J.W. 1983. Yeast vectors with negative selection. Meth. Enzymol. 101:278‐290.
   Sherman, F., Fink, G.R., and Lawrence, C.W. 1979. Methods in Yeast Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
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