Yeast Cloning Vectors and Genes

Victoria Lundblad1

1 University of California, Berkeley, Berkeley, California
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 13.4
DOI:  10.1002/0471142727.mb1304s21
Online Posting Date:  May, 2001
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This unit describes some of the most commonly used yeast vectors, as well as the cloned yeast genes that form the basis for these plasmids. Yeast vectors can be grouped into five general classes, based on their mode of replication in yeast: YIp, YRp, YCp, YEp, and YLp plasmids. With the exception of the YLp plasmids (yeast linear plasmids), all of these plasmids can be maintained in E. coli as well as in S. cerevisiae and thus are referred to as shuttle vectors. The nomenclature of different classes of yeast vectors, as well as details about their mode of replication in yeast are discussed.

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Table of Contents

  • Section II: Yeast Vectors
  • Plasmid Nomenclature
  • Maps of Selected Plasmids and Genes
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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   Bach, M.L. LaCroute, F., and Botstein, D. 1979. Evidence for transcriptional regulation of orotidine‐5′‐phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. U.S.A. 76:386‐390.
   Barnes, D.A. and Thorner, J. 1986. Genetic manipulation of Saccharomyces cerevisiae by use of the LYS2 gene. Molec. Cell. Biol. 6:2828‐2838.
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   Elledge, S.J. and Davis, R.W. 1988. A family of versatile centromeric vectors designed for use in the sectoring‐shuffle mutagenesis assay in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. In press.
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   Hartley, J.L. and Donelson, J.E. 1980. Nucleotide sequence of the yeast plasmid. Nature 286:860‐865.
   Hill, J.E., Myers, A.M., Koerner, T.J., and Tzagoloff, A. 1986. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2:163‐167.
   Ma, H., Kunes, S., Schatz, P.J., and Botstein, D. 1987. Plasmid construction by homologous recombination in yeast. Gene 58:201‐216.
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   Rose, M., Grisaffi, P., and Botstein, D. 1984. Structure and function of the yeast URA3 gene: expression in Escherichia coli. Gene 29:133‐124.
   Rose, M.D., Novick, P., Thomas, J.H., Botstein, D., and Fink, G.R. 1987. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere‐containing shuttle vector. Gene 60:237‐243.
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   Struhl, K., Stinchcomb, D.T., Scherer, S., and Davis, R.W. 1979. High‐frequency transformation of yeast: Autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci. U.S.A. 76:1035‐1039.
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