Yeast Cloning Vectors and Genes

Victoria Lundblad1

1 University of California, Berkeley, Berkeley, California
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 13.4
DOI:  10.1002/0471142727.mb1304s21
Online Posting Date:  May, 2001
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Abstract

This unit describes some of the most commonly used yeast vectors, as well as the cloned yeast genes that form the basis for these plasmids. Yeast vectors can be grouped into five general classes, based on their mode of replication in yeast: YIp, YRp, YCp, YEp, and YLp plasmids. With the exception of the YLp plasmids (yeast linear plasmids), all of these plasmids can be maintained in E. coli as well as in S. cerevisiae and thus are referred to as shuttle vectors. The nomenclature of different classes of yeast vectors, as well as details about their mode of replication in yeast are discussed.

     
 
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Table of Contents

  • Section II: Yeast Vectors
  • Plasmid Nomenclature
  • Maps of Selected Plasmids and Genes
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
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