S. pombe Strain Maintenance and Media

Susan L. Forsburg1

1 Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 13.15
DOI:  10.1002/0471142727.mb1315s64
Online Posting Date:  November, 2003
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Abstract

Fission yeast can be grown and maintained using similar culture methods to those employed for budding yeast. The optimal media for S. pombe is somewhat different than that employed for S. cerevisiae, although budding yeast media can be used if necessary. Good sterile technique is essential to prevent contamination by airborne molds or bacteria.

     
 
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Table of Contents

  • Strain Maintenance and General Guidelines
  • Basic Protocol 1: Preserving Strains by Freezing
  • Media
  • Literature Cited
  • Tables
     
 
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Materials

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Literature Cited

Literature Cited
   Ekwall, K., Nimmo, E.R., Javerzat, J.P., Borgstrom, B., Egel, R., Cranston, G., and Allshire, R. 1996. Mutations in the fission yeast silencing factors clr4+ and rik1+ disrupt the localisation of the chromo domain protein Swi6p and impair centromere function. J. Cell. Sci. 109:2637‐2648.
   Javerzat, J.P., Cranston, G., and Allshire, R.C. 1996. Fission yeast genes which disrupt mitotic chromosome segregation when overexpressed. Nucl. Acids Res. 24:4676‐4683.
   Maundrell, K. 1990. nmt1 of fission yeast: A highly transcribed gene completely repressed by thiamine. J Biol. Chem. 265:10857‐10864.
   Maundrell, K. 1993. Thiamine‐repressible expression vectors prep and prip for fission yeast. Gene 123:127‐130.
   Okazaki, K., Okazaki, N., Kume, K., Jinno, S., Tanaka, K., and Okayama, H. 1990. High‐frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans‐complementation of Schizosaccharomyces pombe. Nucl. Acids Res. 18:6485‐6489.
   Rhind, N. and Russell, P. 1998. The Schizosaccharomyces pombe S‐phase checkpoint differentiates between different types of DNA damage. Genetics 149:1729‐1737.
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