Transgenic Mouse Colony Management

David A. Conner1

1 Harvard Medical School, Cambridge, Massachusetts
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 23.10
DOI:  10.1002/0471142727.mb2310s71
Online Posting Date:  August, 2005
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Abstract

This unit describes strategies and methods used to establish a colony of transgenic mice. Basic mating and genotyping strategies are introduced.

Keywords: mouse colony; genotyping; transgenic

     
 
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Table of Contents

  • Colony Establishment, Expansion, and Maintenance
  • Mouse Identification and Information Management
  • Troubleshooting
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

   Bath, M.L. 2002. Simple and efficient in vitro fertilization with cryopreserved C57BL/6J mouse sperm. Biol. Reprod. 68:19‐23.
   Glenister, P.H. and Rall, W.F. 2000. Cryopreservation and rederivation of embryos and gametes. In Mouse Genetics and Transgenics: A Practical Approach (I.J. Jackson and C.M. Abbot, eds.) pp. 27‐59. Oxford University Press, New York.
   Henikoff, S. 1998. Conspiracy of silence among repeated transgenics. BioEssays 20:532‐535.
   Robertson, G., Garrick, D., Wilson, M., Martin, D.I.K., and Whitlaw, E. 1996. Age‐dependent silencing of globin transgenes in the mouse. Nucleic Acids Res. 24:1465‐1471.
   Shitara, H., Sato, A. Hayashi, J., Mizushima, N., Yonekawa, H., and Taya, C. 2004. Simple method of zygosity identification in transgenic mice by real‐time quantitative PCR. Transgenic Res. 13:191‐194.
   Szczgiel, M.A., Kusakabe, H., Yanagimachi, R., and Whittingham, D.G. 2002. Separation of motile populations of spermatozoa prior to freezing is beneficial for subsequent fertilization in vitro: A study with various mouse strains. Biol. Reprod. 67:287‐292.
   Sztein, J.M., Noble, K., Farley, J.S., and Mobraaten, L.E. 2001. Comparison of permeating and nonpermeating cryoprotectants for mouse sperm cryopreservation. Cryobiology 42:28‐39.
Key References
   Silver, L.M. 1995. Mouse Genetics: Concepts and Applications. Oxford University Press. New York.
  Useful and thorough review of the house mouse and its use in genetics. The text is available on‐line at http://www.informatics.jax.org/mgihome/resources/resources.shtml.
Internet Resources
   http://www.biosupplynet.com
  Search this Web site to obtain a current list of suppliers for animal husbandry–related items.
   http://www.informatics.jax.org
  The Jackson Laboratory informatics Web site provides access to a wide variety of mouse‐related information.
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