Uses of Forward and Reverse Genetics in Mice to Study Gene Function

Carmen A. Argmann1, Andree Dierich2, Johan Auwerx2

1 Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, 2 Institut de Génétique et de Biologie Moléculaire et Cellulaire and Institut Clinique de la Souris, Illkirch
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 29A.1
DOI:  10.1002/0471142727.mb29a01s73
Online Posting Date:  February, 2006
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Abstract

As the focus of human genetics shifts from Mendelian traits to complex diseases, a sophisticated genetic tool kit—with space for genetics (classical, molecular, statistical, and quantitative), metabolics, proteomics, bioinformatics, and mathematics—is required to elucidate their multifactorial traits and regulatory processes. Importantly, mouse resources optimized to study the actions of isolated genetic loci on a fixed background are insufficient on their own for studying intact polygenic networks and genetic interactions, and researchers must work in the context of experimental model systems that optimally mimic the genetic structure of human populations. The success of such phenogenomic approaches depend on the efficacy by which specific mutations (gene targeting) and variability (recombinant inbreeding) can be introduced into the mouse genome, and on the optimization of phenotyping analyses of the mutant mouse lines. This unit describes the basic genetic approaches used to in the study of mouse model systems.

Keywords: GEMMs; GRPs; phenotyping; forward genetics; reverse genetics; mouse

     
 
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Table of Contents

  • Reverse Genetics
  • Forward Genetics
  • Polygenic Diseases and Genetic Reference Panels
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

Videos

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Internet Resources
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