Collection of Blood and Plasma from the Mouse

Carmen A. Argmann1, Johan Auwerx2

1 Institut de Genetique et de Biologie, Moleculaire et Cellulaire, Illkirch, France, 2 Institut de Genetique et de Biologie, Moleculaire et Cellulaire and Institut, Clinique de la Souris, Illkirch, France
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 29A.3
DOI:  10.1002/0471142727.mb29a03s75
Online Posting Date:  August, 2006
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

There are many elements in plasma that can act as surrogate markers of the physiological well‐being of a mouse, thus making the collection of blood and plasma a general technique with many applications in mouse phenotyping. For example, the presence of certain enzymes in plasma can serve as markers of tissue toxicity (AST, ALT) and general function, and the more sophisticated lipid and lipoprotein profile tests (cholesterol, LDL) can point to dyslipidemias. As many of the tests available to measure these parameters have been adapted to automated systems in a high‐throughput fashion, they have become part of the first line of screening protocols in mouse phenotyping. In this section, general techniques associated with collection and processing of blood are described.

Keywords: blood draw; serum; plasma

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • Commentary
  • Literature Cited
  • Figures
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

Basic Protocol 1:

  Materials
  • Mouse
  • Hematocrit sealing compound (e.g., http://www.vitrex.dk)
  • Alcohol‐soaked cotton pads
  • Sterile gauze
  • Surgical blade, no. 10 or 11
  • Microhematocrit capillary tubes (with or without Na‐heparin or EDTA, depending on analyte and plasma versus serum)
  • 5‐ml polypropylene centrifuge tubes
  • Refrigerated centrifuge
  • 100‐µl Hamilton syringe
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

Literature Cited
   Coligan, J.E., Bierer, B.E., Margulies, D.H., She vach, E.M., and Strober, W. 2006. Current Protocols in Immunology. John Wiley & Sons, Hoboken, N.J.
   Hedrich, H. (ed.) 2004. The Laboratory Mouse. Elsevier Academic Press, New York.
  Hoff, J. 2000. Methods of blood collection in the mouse. Lab. Animal 29:47‐53.
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library