Commonly Used Reagents and Equipment

David D. Moore1

1 Massachusetts General Hospital, Boston, Massachusetts
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Appendix 2
DOI:  10.1002/0471142727.mba02s35
Online Posting Date:  May, 2001
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Abstract

This appendix lists recipes for common reagents and also describes equipment that is typically found in a molecular biology laboratory.

    Buffers and Stock Solutions

  • Acid precipitation solution

  • Ammonium acetate, 10 M

  • BBS (BES‐buffered solution), 2×

  • CaCl2, 1 M

  • Denhardt solution, 100×

  • Dithiothreitol (DTT), 1 M

  • EDTA (ethylenediamine tetraacetic acid), 0.5 M (pH 8.0)

  • Ethidium bromide, 10 mg/ml

  • HBSS (Hanks balanced salt solution)

  • HCl, 1 M

  • HeBS (HEPES‐buffered saline) solution, 2×

  • KCl, 1 M

  • MgCl2, 1 M

  • MgSO4, 1 M

  • MOPS buffer

  • NaCl, 5 M

  • NaOH, 10 M

  • PBS (phosphate‐buffered saline)

  • Potassium acetate buffer, 0.1 M

  • Potassium phosphate buffer, 0.1 M

  • SDS electrophoresis buffer, 5×

  • SED (standard enzyme diluent)

  • Sodium acetate, 3 M

  • Sodium acetate buffer, 0.1 M

  • Sodium phosphate buffer, 0.1 M

  • SSC (sodium chloride/sodium citrate), 20×

  • STE buffer

  • TAE (Tris/acetate/EDTA) electrophoresis buffer

  • TBE (Tris/borate/EDTA) electrophoresis buffer

  • TE (Tris/EDTA) buffer

  • TEA (triethanolamine) solution

  • TEN (Tris/EDTA/NaCl) solution

  • TM buffer, 10×

  • Tris‐buffered saline (TBS)

  • Tris⋅Cl [tris(hydroxymethyl)aminomethane], 1 M

  • TTBS (Tween 20/TBS)

     
 
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Table of Contents

  • Literature Cited
  • Tables
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
   Chemical Rubber Company (CRC). 1975. CRC Handbook of Biochemistry and Molecular Biology, Physical and Chemical Data, 3rd ed., Vol. 1. CRC Press, Boca Raton, Fla.
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