Silanizing Glassware

Brian Seed1

1 Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Appendix 3B
DOI:  10.1002/0471142727.mba03bs28
Online Posting Date:  May, 2001
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Abstract

This unit presents a procedure to silanize (siliconize) glassware to prevent adsorption of solute to the glass surface or to increase its hydrophobicity. This is particularly important when dealing with low concentrations of particularly “sticky” solutes such as single‐stranded nucleic acids or proteins.

     
 
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Table of Contents

  • Commentary
     
 
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Materials

Basic Protocol 1:

  Materials
  • Chlorotrimethylsilane or dichlorodimethylsilane
  • Vacuum pump
  • Desiccator, equipped with a valve
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Figures

Videos

Literature Cited

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