Introduction of Restriction Enzyme Recognition Sequences by Silent Mutation

Raj Shankarappa1

1 University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Appendix 3E
DOI:  10.1002/0471142727.mba03es35
Online Posting Date:  May, 2001
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Abstract

This unit describes silent mutations and provides tables of translation sequences for silent mutagenesis based on six‐ and eight‐base restriction enzymes.

     
 
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Table of Contents

  • Literature Cited
  • Tables
     
 
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Materials

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Literature Cited

Literature Cited
   Shankarappa, B., Sirko, D.A., and Ehrlich, G.D. 1992. A general method for the identification of regions suitable for site‐directed silent mutagenesis. BioTechniques 12:382‐384.
   Shankarappa, B., Balachandran, R., Gupta, P., and Ehrlich, G.D. 1992b. Introduction of multiple restriction enzyme sites by in vitro mutagenesis using the polymerase chain reaction. PCR Method. Appl. 1:277‐278.
   Shankarappa, B., Vijayananda, K., and Ehrlich, G.D. 1992c. SILMUT: A computer program for the identification of regions compatible for the introduction of restriction enzyme sites by site‐directed silent mutagenesis. BioTechniques 12:882‐884.
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