Preparing for Biofilm Studies in the Field

Robert J.C. McLean1, Thomas R. Simpson1

1 Department of Biology, Texas State University‐San Marcos, San Marcos, Texas
Publication Name:  Current Protocols in Microbiology
Unit Number:  Unit 1B.4
DOI:  10.1002/9780471729259.mc01b04s10
Online Posting Date:  August, 2008
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Abstract

In their natural environments, microorganisms are under constant environmental selection to form biofilms. Using aquatic biofilms as an example, this unit illustrates general concepts in field biology and practical suggestions for designing, conducting, and analyzing biofilm experiments at varying distances from the laboratory. The unit also addresses an example of a special situation (space flight) where experimentation must be done by proxy through another individual or machine. Curr. Protoc. Microbiol. 10:1B.4.1‐1B.4.14. © 2008 by John Wiley & Sons, Inc.

Keywords: biofilm; bacteria; field studies

     
 
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Table of Contents

  • Introduction
  • Technique Refinement and Testing
  • General Aspects of Field Research
  • Field Studies in Proximity to Laboratory Facilities
  • Collection of Biofilm Samples Remote from the Laboratory
  • Sampling by Proxy
  • Conclusion
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Cassanto, V.A. and Wood, B.J. 1998. Student experiments in space: A private‐sector model. Earth Space Rev. 7:25‐28.
   Dunn, K.A., McLean, R.J.C., Upchurch, G.R. Jr., and Folk, R.L. 1997. Enhancement of leaf fossilization potential by bacterial biofilms. Geology 25:1119‐1122.
   Friend, M. and Franson, J.C. 1999. Field Manual of Wildlife Diseases: General Field Procedures and Diseases of Birds. Biological Resources Division, U.S. Geological Survey, Washington, D.C.
   McLean, R.J.C., Cassanto, J.M., Barnes, M.B., and Koo, J. 2001. Bacterial biofilm formation under microgravity conditions. FEMS Microbiol. Lett. 195:115‐119.
   McLean, R.J.C., Welsh, A.K., and Casasanto, V.A. 2006. Microbial survival in space shuttle crash. Icarus 181:323‐325.
   Whiteley, M., Ott, J.R., Weaver, E.A., and McLean, R.J.C. 2001. Effects of community composition and growth rate on aquifer biofilm bacteria and their susceptibility to Betadine disinfection. Environ. Microbiol. 3:43‐52.
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