Growth Conditions for Plant Virus–Host Studies

Jian‐Zhong Liu1, Kristy Richerson2, Richard S. Nelson3

1 University of California‐Berkeley, Albany, California, 2 Andreae Team, Inc., Ardmore, Oklahoma, 3 Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma
Publication Name:  Current Protocols in Microbiology
Unit Number:  Unit 16A.1
DOI:  10.1002/9780471729259.mc16a01s14
Online Posting Date:  August, 2009
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Plant virus replication and spread require specific interactions between the obligate organism, the virus, and its host, the plant. Both virus and plant factors, whether they are involved in virus accumulation or in defense against virus accumulation, require specific environmental conditions to maximize their activity. In order to understand how virus and plant factors function, it is therefore necessary to understand and control the environment that influences their activity. Temperature, light, moisture, air movement, soil status, and plant nutrition are the major environmental factors that influence a virus' accumulation in its host. This unit discusses each of these environmental factors, documenting their influence on virus accumulation in the plant, and discusses methods to best control and monitor their variation. Controlling the environment will limit experimental variation and lead to more meaningful and reproducible experimental results. Curr. Protoc. Microbiol. 14:16A.1.1‐16A.1.16. © 2009 by John Wiley & Sons, Inc.

Keywords: plant viruses; environment; growth conditions; greenhouses; growth chambers

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Table of Contents

  • Introduction
  • An Overview of Environmental Factors that Influence Virus Accumulation and Disease Development
  • Greenhouse and Growth Chamber Settings
  • Insect Pest, Alternate Pathogen, and Non‐Pathogen Control
  • Acknowledgements
  • Literature Cited
  • Tables
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Literature Cited

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